Thermodynamic parameters for LDH in crude homogenate of Gadus morhua, which were calculated for the temperature dependence ofν max
| Tissue . | TA (°C) . | Ldh-B phenotype . | Ea (kJ mol—1) . | r2 . | ΔH(TA) (kJ mol—1) . | ΔS (kJ mol—1 K—1) . | ΔG(TA) (kJ mol—1) . | N . | n . |
|---|---|---|---|---|---|---|---|---|---|
| Muscle | 4 | a/a | 53.76±0.74 | 0.972±0.008 | 51.45±0.74 | —14.84±2.57 | 55.56±0.03 | 5 | 1016 |
| a/b | 56.76±1.96 | 0.981±0.005 | 54.46±1.96 | —4.42±6.79 | 55.68±0.08 | 7 | 1430 | ||
| b/b | 58.64±1.33 | 0.991±0.002 | 56.34±1.33 | 2.11±4.60 | 55.76±0.05 | 9 | 1758 | ||
| Pooled | 56.55±0.97 | 0.983±0.003 | 54.25±0.97 | —5.14±3.38 | 55.67±0.04 | 21 | 4204 | ||
| 12 | a/a | 46.55±1.26 | 0.892±0.049 | 44.18±1.26 | —40.07±4.38 | 55.60±0.01 | 3 | 566 | |
| a/b | 48.94±1.63 | 0.947±0.013 | 46.57±1.63 | —31.79±5.66 | 55.63±0.02 | 6 | 1199 | ||
| b/b | 46.87±1.88 | 0.966±0.008 | 44.50±1.88 | —38.96±6.51 | 55.60±0.02 | 6 | 1221 | ||
| Pooled | 47.63±1.01 | 0.944±0.012 | 45.26±1.01 | —36.31±3.49 | 55.61±0.01 | 15 | 2986 | ||
| Δ | 8.99 | ||||||||
| Liver | 4 | a/a | 57.96±4.85 | 0.956±0.025 | 55.65±4.85 | —16.87±3.59 | 55.73±0.19 | 5 | 1032 |
| a/b | 47.75±1.44 | 0.981±0.003 | 45.44±1.44 | —35.68±4.99 | 55.33±0.06 | 7 | 1435 | ||
| b/b | 55.13±1.31 | 0.972±0.006 | 52.83±1.31 | —10.06±4.55 | 55.62±0.05 | 9 | 1799 | ||
| Pooled | 53.34±1.57 | 0.971±0.006 | 51.04±1.57 | —20.39±3.75 | 55.55±0.06 | 21 | 4266 | ||
| 12 | a/a | 49.01±4.33 | 0.947±0.022 | 46.64±4.33 | —31.53±15.01 | 55.63±0.05 | 3 | 588 | |
| a/b | 47.18±1.83 | 0.958±0.008 | 44.81±1.83 | —37.88±6.36 | 55.61±0.02 | 6 | 1181 | ||
| b/b | 47.76±2.58 | 0.960±0.012 | 45.39±2.58 | —35.88±8.97 | 55.61±0.03 | 6 | 1144 | ||
| Pooled | 47.78±1.42 | 0.956±0.007 | 45.41±1.42 | —35.81±4.91 | 55.61±0.02 | 15 | 2913 | ||
| Δ | 5.63 |
| Tissue . | TA (°C) . | Ldh-B phenotype . | Ea (kJ mol—1) . | r2 . | ΔH(TA) (kJ mol—1) . | ΔS (kJ mol—1 K—1) . | ΔG(TA) (kJ mol—1) . | N . | n . |
|---|---|---|---|---|---|---|---|---|---|
| Muscle | 4 | a/a | 53.76±0.74 | 0.972±0.008 | 51.45±0.74 | —14.84±2.57 | 55.56±0.03 | 5 | 1016 |
| a/b | 56.76±1.96 | 0.981±0.005 | 54.46±1.96 | —4.42±6.79 | 55.68±0.08 | 7 | 1430 | ||
| b/b | 58.64±1.33 | 0.991±0.002 | 56.34±1.33 | 2.11±4.60 | 55.76±0.05 | 9 | 1758 | ||
| Pooled | 56.55±0.97 | 0.983±0.003 | 54.25±0.97 | —5.14±3.38 | 55.67±0.04 | 21 | 4204 | ||
| 12 | a/a | 46.55±1.26 | 0.892±0.049 | 44.18±1.26 | —40.07±4.38 | 55.60±0.01 | 3 | 566 | |
| a/b | 48.94±1.63 | 0.947±0.013 | 46.57±1.63 | —31.79±5.66 | 55.63±0.02 | 6 | 1199 | ||
| b/b | 46.87±1.88 | 0.966±0.008 | 44.50±1.88 | —38.96±6.51 | 55.60±0.02 | 6 | 1221 | ||
| Pooled | 47.63±1.01 | 0.944±0.012 | 45.26±1.01 | —36.31±3.49 | 55.61±0.01 | 15 | 2986 | ||
| Δ | 8.99 | ||||||||
| Liver | 4 | a/a | 57.96±4.85 | 0.956±0.025 | 55.65±4.85 | —16.87±3.59 | 55.73±0.19 | 5 | 1032 |
| a/b | 47.75±1.44 | 0.981±0.003 | 45.44±1.44 | —35.68±4.99 | 55.33±0.06 | 7 | 1435 | ||
| b/b | 55.13±1.31 | 0.972±0.006 | 52.83±1.31 | —10.06±4.55 | 55.62±0.05 | 9 | 1799 | ||
| Pooled | 53.34±1.57 | 0.971±0.006 | 51.04±1.57 | —20.39±3.75 | 55.55±0.06 | 21 | 4266 | ||
| 12 | a/a | 49.01±4.33 | 0.947±0.022 | 46.64±4.33 | —31.53±15.01 | 55.63±0.05 | 3 | 588 | |
| a/b | 47.18±1.83 | 0.958±0.008 | 44.81±1.83 | —37.88±6.36 | 55.61±0.02 | 6 | 1181 | ||
| b/b | 47.76±2.58 | 0.960±0.012 | 45.39±2.58 | —35.88±8.97 | 55.61±0.03 | 6 | 1144 | ||
| Pooled | 47.78±1.42 | 0.956±0.007 | 45.41±1.42 | —35.81±4.91 | 55.61±0.02 | 15 | 2913 | ||
| Δ | 5.63 |
TA, acclimation temperature; Ea,apparent Arrhenius activation energy calculated on the base of temperature dependence of νmax (equation 4); r2, squared Pearson correlation coefficient for the slope of the Arrhenius plot;Δ H(TA), change of activation enthalpy calculated for corresponding acclimation temperature; ΔS,change of activation entropy; ΔG(TA),change of Gibbs free energy calculated for corresponding acclimation temperature; N, number of analysed fishes; n, total number of enzymatic reactions, which were read at different pyruvate concentrations and different temperatures of assay to calculate mean Ea;Δ, difference between ΔH for different acclimation temperatures. Values are given as means ± s.e.m.