Table 1.

PCR primer sequences (5′→3′)

SequenceForwardReverse
S1MHC* CGAAACCAATGCTCTGAAGAACACC GTAAGCATCAAGTACCGTCCAAGG 
S2 MHC CGCCTCGAAGTTGAGGGTAAT ACAGGTGTTACTTGTCGGTGTCGG 
S1 Tm S2 Tm forward primer AAATAGGTTACAGGCCAGCTGAGG 
S2 Tm TCGACAGGCTCGAAGATGAACTTG AGCTTCGATAACGCTCACTACCAC 
SequenceForwardReverse
S1MHC* CGAAACCAATGCTCTGAAGAACACC GTAAGCATCAAGTACCGTCCAAGG 
S2 MHC CGCCTCGAAGTTGAGGGTAAT ACAGGTGTTACTTGTCGGTGTCGG 
S1 Tm S2 Tm forward primer AAATAGGTTACAGGCCAGCTGAGG 
S2 Tm TCGACAGGCTCGAAGATGAACTTG AGCTTCGATAACGCTCACTACCAC 

MHC, myosin heavy chain; Tm, tropomyosin; S1, slow-twitch muscle; S2, slow-tonic muscle.

*

Primers for S1 MHC are the same as used by Medler and Mykles(2003)

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