The Company of Biologists

Table 2.

Primers used for RT-PCR of MHC isoforms

MHC-2A

for 5′AGCCGGGAGGTTCACACAAA3′

rev 5′CTTTGCAATAGGGTAGGATAGGC3′

MHC-2B

for 5′TGAAAATGAACAGAAGCGCAATG3′

rev 5′TTTCCTTGACATCACATGACATATAAC3′

MHC-2X

for 5′TGAAGAAGAACCTGGAGCAGACG3′

rev 5′CATTAAGTACAAAACAGAGTGACAAAGATT3′

MHC-slow

for 5′TGGAGCGCATGAAGAAGAACA3′

rev 5′AGTGCTTTATTCTGCTTCCTCCAA3′

for, forward; rev, reverse.