The Company of Biologists

Table 1.

ATP, ADP, total adenylates (Ad_tot), phosphocreatine (PCr),creatine (Cr), total creatine (Cr_tot), sum of high-energy phosphates (HEP_tot=ATP+0.5×ADP+PCr), phosphorylation potential (PCr/Cr²) and lactate in cardiac tissue from turtle and trout under oxygenation and after 40 and 70 min of severe hypoxia

O₂/N₂	Rest/stim.	ATP (μmol l^-1 g^-1)	ADP (μmol l^-1 g^-1)	PCr (μmol l^-1 g^-1)	Cr	Ad_tot (μmol l^-1 g^-1)	Cr_tot (μmol l^-1 g^-1)	HEP_tot (μmol l^-1 g^-1)	PCr/Cr² (μmol l^-1 g^-1)	Muscle lactate (μmol l^-1 g^-1)
Turtle
Oxygenated	Rest	2.51±0.32	0.51±0.02	4.83±0.31^a	3.31±0.26^a	3.18±0.34	8.14±0.52	7.60±0.60^a	0.47±0.06^a	1.15±0.21^a
Hypoxia 40 min	Rest	2.35±0.28	0.55±0.03	3.51±0.33^b	4.39±0.38^a,b,c	3.04±0.30	7.90±0.63	6.14±0.59^a,b	0.19±0.02^b	5.40±1.17^b
Hypoxia 70 min	Rest	1.88±0.27	0.50±0.03	3.38±0.69^b	4.04±0.45^a,b	2.50±0.31	7.42±1.13	5.51±0.96^b,c	0.20±0.01^b	3.48±0.31^b,c
Oxygenated	Stim.	2.39±0.26	0.53±0.07	3.96±0.35^b	3.73±0.29^a	3.08±0.34	7.68±0.58	6.62±0.58^a,b	0.30±0.04^c	3.29±0.34^a,c
Hypoxia 40 min	Stim.	1.74±0.14	0.52±0.04	1.78±0.21^c	5.17±0.38^b,c	2.37±0.17	6.96±0.48	3.78±0.21^c	0.07±0.02^d	11.31±0.62^d
Hypoxia 70 min	Stim.	1.65±0.16	0.52±0.02	2.93±0.98^d	5.40±0.71^c	2.28±0.17	8.33±1.54	4.84±1.03^b,c	0.10±0.02^b,d	12.85±1.00^d
Trout
Oxygenated	Rest	2.56±0.21^a	0.59±0.05^a	7.97±0.87^a	4.04±0.49^a	3.38±0.27^a	12.0±1.01^a,c	10.83±1.04^a	0.70±0.15^a	5.08±0.70^a
Hypoxia 40 min	Rest	2.21±0.21^a	0.98±0.07^b	2.60±0.48^b	7.71±0.49^b	3.47±0.26^a	10.3±0.86^a,b,c	5.31±0.70^b,c	0.04±0.01^b	8.83±1.06^b
Hypoxia 70 min	Rest	2.38±0.29^a	0.84±0.09^b,c	3.00±0.39^b	7.42±0.76^b	3.46±0.39^a	10.42±0.97^a,b,c	5.81±0.67^b	0.06±0.01^b	6.91±1.06^a,b
Oxygenated	Stim.	2.63±0.14^a	0.68±0.04^a,c	8.10±0.61^a	4.82±0.41^a	3.55±0.17^a	12.9±0.80^a	11.06±0.74^a	0.42±0.07^c	6.67±0.64^a,b
Hypoxia 40 min	Stim.	1.30±0.24^b	0.76±0.06^c	0.96±0.18^b	7.59±0.49^b	2.32±0.31^b	8.54±0.61^b	2.64±0.44^c	0.02±0.003^b	16.52±1.33^c
Hypoxia 70 min	Stim.	1.46±0.21^b	0.79±0.04^c	0.91±0.16^b	8.69±0.49^b	2.56±0.22^b	9.60±0.47^c	2.77±0.36^c	0.01±0.003^b	15.62±3.13^c

O₂/N₂	Rest/stim.	ATP (μmol l^-1 g^-1)	ADP (μmol l^-1 g^-1)	PCr (μmol l^-1 g^-1)	Cr	Ad_tot (μmol l^-1 g^-1)	Cr_tot (μmol l^-1 g^-1)	HEP_tot (μmol l^-1 g^-1)	PCr/Cr² (μmol l^-1 g^-1)	Muscle lactate (μmol l^-1 g^-1)
Turtle
Oxygenated	Rest	2.51±0.32	0.51±0.02	4.83±0.31^a	3.31±0.26^a	3.18±0.34	8.14±0.52	7.60±0.60^a	0.47±0.06^a	1.15±0.21^a
Hypoxia 40 min	Rest	2.35±0.28	0.55±0.03	3.51±0.33^b	4.39±0.38^a,b,c	3.04±0.30	7.90±0.63	6.14±0.59^a,b	0.19±0.02^b	5.40±1.17^b
Hypoxia 70 min	Rest	1.88±0.27	0.50±0.03	3.38±0.69^b	4.04±0.45^a,b	2.50±0.31	7.42±1.13	5.51±0.96^b,c	0.20±0.01^b	3.48±0.31^b,c
Oxygenated	Stim.	2.39±0.26	0.53±0.07	3.96±0.35^b	3.73±0.29^a	3.08±0.34	7.68±0.58	6.62±0.58^a,b	0.30±0.04^c	3.29±0.34^a,c
Hypoxia 40 min	Stim.	1.74±0.14	0.52±0.04	1.78±0.21^c	5.17±0.38^b,c	2.37±0.17	6.96±0.48	3.78±0.21^c	0.07±0.02^d	11.31±0.62^d
Hypoxia 70 min	Stim.	1.65±0.16	0.52±0.02	2.93±0.98^d	5.40±0.71^c	2.28±0.17	8.33±1.54	4.84±1.03^b,c	0.10±0.02^b,d	12.85±1.00^d
Trout
Oxygenated	Rest	2.56±0.21^a	0.59±0.05^a	7.97±0.87^a	4.04±0.49^a	3.38±0.27^a	12.0±1.01^a,c	10.83±1.04^a	0.70±0.15^a	5.08±0.70^a
Hypoxia 40 min	Rest	2.21±0.21^a	0.98±0.07^b	2.60±0.48^b	7.71±0.49^b	3.47±0.26^a	10.3±0.86^a,b,c	5.31±0.70^b,c	0.04±0.01^b	8.83±1.06^b
Hypoxia 70 min	Rest	2.38±0.29^a	0.84±0.09^b,c	3.00±0.39^b	7.42±0.76^b	3.46±0.39^a	10.42±0.97^a,b,c	5.81±0.67^b	0.06±0.01^b	6.91±1.06^a,b
Oxygenated	Stim.	2.63±0.14^a	0.68±0.04^a,c	8.10±0.61^a	4.82±0.41^a	3.55±0.17^a	12.9±0.80^a	11.06±0.74^a	0.42±0.07^c	6.67±0.64^a,b
Hypoxia 40 min	Stim.	1.30±0.24^b	0.76±0.06^c	0.96±0.18^b	7.59±0.49^b	2.32±0.31^b	8.54±0.61^b	2.64±0.44^c	0.02±0.003^b	16.52±1.33^c
Hypoxia 70 min	Stim.	1.46±0.21^b	0.79±0.04^c	0.91±0.16^b	8.69±0.49^b	2.56±0.22^b	9.60±0.47^c	2.77±0.36^c	0.01±0.003^b	15.62±3.13^c

Values are means ± s.e.m. (N=6 for all groups except oxygenated trout preparations for which N=11/12). Dissimilar letters indicate significant differences due to treatments for turtle and trout, respectively, and differences between turtle and trout are indicated by presenting the values in bold.

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