Effects of temperature and rapid cold-hardening on courtship parameters in D. melanogaster
| Mating temperature . | Treatment group (N) . | Number of courtship events . | Duration of individual courtship events (min) . | Courtship index (%) . |
|---|---|---|---|---|
| 16°C | Control (22) | 0.9±0.2a | 9.6±0.8a | 20.6±5.7a |
| 16°C | RCH (20) | 1.6±0.3a,b | 19.1±3.4b | 53.3±8.4b |
| 23°C | Control (20) | 3.0±0.8b,c | 7.9±1.2a | 40.8±6.3a,b |
| 23°C | RCH (19) | 6.6±2.1c | 4.9±0.7a | 52.3±3.9b |
| Mating temperature . | Treatment group (N) . | Number of courtship events . | Duration of individual courtship events (min) . | Courtship index (%) . |
|---|---|---|---|---|
| 16°C | Control (22) | 0.9±0.2a | 9.6±0.8a | 20.6±5.7a |
| 16°C | RCH (20) | 1.6±0.3a,b | 19.1±3.4b | 53.3±8.4b |
| 23°C | Control (20) | 3.0±0.8b,c | 7.9±1.2a | 40.8±6.3a,b |
| 23°C | RCH (19) | 6.6±2.1c | 4.9±0.7a | 52.3±3.9b |
Flies in the rapid cold-hardening (RCH) group were subjected to a 2-h 16°C pre-treatment prior to mating trials. Control flies were held at 23°C until immediately before transfer to the mating temperature. Courtship index is defined as the percentage of the 1-h mating trial that a pair spends in reproductive behavior. Values are given as means ± s.e.m.; N=number of pairs. Values within columns are statistically different if they do not share a common letter(Kruskal—Wallis, multiple comparisons post-hoc tests, P<0.05).