Summary of data from the riboprobe/22C10 double label and double riboprobe experiments
| . | Head segments . | . | . | . | . | . | . | |
|---|---|---|---|---|---|---|---|---|
| . | Dorsal ganglion . | Terminal ganglion . | T1 . | T2 or T3 lateral cluster . | T2 or T3 ventral cluster . | A1 or A2 ventral group . | A8/A9 . | |
| Total neurons in ganglion or cluster per hemi-segment | ∼20 | 20+ | ND | 11 | 15 | 8 | ND | |
| Gyc-88E | 0 | 1 | 2 | 1 | 1 | 1 | 6 | |
| Gyc-89Db | 4-5 | 2-5 | 2 | 1 | 1 | 1 | 6 | |
| Both probes | 4-5 | 2-5 | 2 | 1 | 1 | 1 | 6 | |
| Identity | ND | ND | ND | les | ves | v'td | ND | |
| . | Head segments . | . | . | . | . | . | . | |
|---|---|---|---|---|---|---|---|---|
| . | Dorsal ganglion . | Terminal ganglion . | T1 . | T2 or T3 lateral cluster . | T2 or T3 ventral cluster . | A1 or A2 ventral group . | A8/A9 . | |
| Total neurons in ganglion or cluster per hemi-segment | ∼20 | 20+ | ND | 11 | 15 | 8 | ND | |
| Gyc-88E | 0 | 1 | 2 | 1 | 1 | 1 | 6 | |
| Gyc-89Db | 4-5 | 2-5 | 2 | 1 | 1 | 1 | 6 | |
| Both probes | 4-5 | 2-5 | 2 | 1 | 1 | 1 | 6 | |
| Identity | ND | ND | ND | les | ves | v'td | ND | |
Diagrams of the Drosophila peripheral nervous system by Younossi-Hartenstein and Hartenstein(1997), Bodmer and Jan (1986),Brewster and Bodmer (1995) and Stocker (1994) were used to identify the neuron cluster or ganglion location of neurons that expressed Gyc-88E and Gyc-89Db in stage 17 embryos. Numbers refer to each side of the animal and were obtained by examining at least 50 embryos that were labeled with a riboprobe for each guanylyl cyclase, either individually or used together, and the 22C10 antibody. Embryos treated with both riboprobes stained the same number of cells in the thoracic and abdominal segments, indicating that Gyc-88E and Gyc-89Db are co-expressed in the same neurons. ND, not determined.