Results of fitting an exponential function of the form V=V0+(Vm-V0)(1-e-kt) to experimental data ofFig. 1
| Treatment . | Vm (10-9 cm3) . | t1/2 (min) . |
|---|---|---|
| 120 mosmoll-1 | 3.46±0.24 | 7.54±1.29 |
| 180 mosmoll-1 | 2.00±0.12 | 5.44±1.60 |
| 180 mosmoll-1 (CFM) | 2.03±0.18 | 7.92±4.05 |
| ALA-AOA | 1.63±0.08 | 11.93±7.07 |
| 503 mosmoll-1 | 0.90±0.07 | 8.41±1.54 |
| Treatment . | Vm (10-9 cm3) . | t1/2 (min) . |
|---|---|---|
| 120 mosmoll-1 | 3.46±0.24 | 7.54±1.29 |
| 180 mosmoll-1 | 2.00±0.12 | 5.44±1.60 |
| 180 mosmoll-1 (CFM) | 2.03±0.18 | 7.92±4.05 |
| ALA-AOA | 1.63±0.08 | 11.93±7.07 |
| 503 mosmoll-1 | 0.90±0.07 | 8.41±1.54 |
Values of parameters ± S.E. (standard error of the fit).
V0 and Vm denote initial and maximal/minimal total volume (V), respectively. The value of t1/2 (the time necessary to achieve 50% of Vm) was calculated as follows: t1/2=ln2/k, where k denotes a constant. ALA-AOA denotes an isosmotic medium in the presence of 10 mmoll-1 L-alanine plus 2.5 mmoll-1 aminooxyacetic acid. CFM denotes experiments with hepatocytes that had been isolated in collagenase-free medium (see Materials and methods).