Treatment . | 5′ (×103 d.p.m.) . | 3′ (×103 d.p.m.) . | Initiation per elongation* . | Percent new initiation(%)† . | N . |
---|---|---|---|---|---|
Control (normoxic, pH 7.9) | 399±23 | 267±35 | 7.6 | 76.8±8.8 | 9 |
Normoxia, pH 6.4 | 89±0.4 | 10±0.7 | 0.91 | 31.3±5.2‡ | 7 |
In vitro anoxia, pH 6.4 | 16±2.4 | 19±0.4 | 0.96 | 32.9±5.6‡ | 4 |
In vitro anoxia, pH 7.9 | 36±1.9 | 20±1.0 | 7.5 | 78.8±4.1 | 3 |
In vivo anoxia | 170±7.0 | 82±4.7 | 15 | 88.6±1.6 | 4 |
Treatment . | 5′ (×103 d.p.m.) . | 3′ (×103 d.p.m.) . | Initiation per elongation* . | Percent new initiation(%)† . | N . |
---|---|---|---|---|---|
Control (normoxic, pH 7.9) | 399±23 | 267±35 | 7.6 | 76.8±8.8 | 9 |
Normoxia, pH 6.4 | 89±0.4 | 10±0.7 | 0.91 | 31.3±5.2‡ | 7 |
In vitro anoxia, pH 6.4 | 16±2.4 | 19±0.4 | 0.96 | 32.9±5.6‡ | 4 |
In vitro anoxia, pH 7.9 | 36±1.9 | 20±1.0 | 7.5 | 78.8±4.1 | 3 |
In vivo anoxia | 170±7.0 | 82±4.7 | 15 | 88.6±1.6 | 4 |
The ratio of 2.52Y to 0.55X gives the d.p.m. due to initiation and elongation in the 5′ fragment, where X and Y are calculated as in the legend of Fig. 1. Note that specific activity in the 5′ fragment from elongation is half that due to initiation, so initiation per elongation is halved to calculate percent new initiation. The number of separate determinations (N) for percent new initiation is also shown.
Reported as mean ± S.E.M.
Significant difference relative to control (P<0.05).