The Company of Biologists

Table 2.

Transcriptional rates and total UTP incorporation decrease in isolated mitochondria from Artemia franciscana embryos when incubated under anoxia or pH 6.4

Treatments	Transcription rate (with all initiation)^{^*}	[³²P]UTP incorporation(%)^{^†}	N
Normoxia, pH 7.9	3197±94	100	9
Anoxia in vivo and in vitro, pH 6.4	361±108	23	3
Anoxia in vivo and in vitro, pH 7.9	3015±54	48	6
Normoxia, pH 6.4	837±45	41	6

Treatments	Transcription rate (with all initiation)^{^*}	[³²P]UTP incorporation(%)^{^†}	N
Normoxia, pH 7.9	3197±94	100	9
Anoxia in vivo and in vitro, pH 6.4	361±108	23	3
Anoxia in vivo and in vitro, pH 7.9	3015±54	48	6
Normoxia, pH 6.4	837±45	41	6

Transcription rates were calculated across the linear portions of run-on assays. Data are expressed as means ± S.E.M. Units for rates are nmol UTP incorporated mg mitochondrial protein^-1 h^-1.

^†

Expressed as percentage incorporation relative to controls (normoxia, pH 7.9) over the 30 min assay.

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