The Company of Biologists

Table 1.

Locations and sequences of primers used in ribonuclease protection assays and primer extension reactions

Primer name	Genomic location^{^*}	Sequence^{^†}
RPA antisense clones
12S 5′ forward	13 996-13 977	5′ AGTTTGATTCTAGCTAGTTT (H)
12S 5′ reverse	13 715-13 696	5′ AGGGTATCTAATCCTAGTTC (L)
12S 3′ forward	13 399-13 380	5′ GTCTATCAACCTGAAGGAGG (H)
12S 3′ reverse	13 309-13 290	5′ TTTCAAGATACACCTTCCGG (L)
Footprinting
LSP 1	15 664-15 683	5′ AAGAATTATATGTTTGATAA (H)
LEU 1	12 125-12 144	5′ GTAAGCCAGGTTAGTTTCTA (H)
HSP 1	14 061-14 080	5′ CTTTGTCCGTTACTATTACT (H)
HSP 2	13 890-13 909	5′ ACTAGCTAGAATCAAACTTT (L)
HSP 3	14 171-14 190	5′ TCAAAGCAGTCTCCCCGGCG (H)
HSP 4	14 250-14 269	5′ ATTTAAATGTGGAAAAATAC (H)
HSP 5	13 921-13 940	5′ TCACTGCACTTCTAACCTAT (L)

Primer name	Genomic location^{^*}	Sequence^{^†}
RPA antisense clones
12S 5′ forward	13 996-13 977	5′ AGTTTGATTCTAGCTAGTTT (H)
12S 5′ reverse	13 715-13 696	5′ AGGGTATCTAATCCTAGTTC (L)
12S 3′ forward	13 399-13 380	5′ GTCTATCAACCTGAAGGAGG (H)
12S 3′ reverse	13 309-13 290	5′ TTTCAAGATACACCTTCCGG (L)
Footprinting
LSP 1	15 664-15 683	5′ AAGAATTATATGTTTGATAA (H)
LEU 1	12 125-12 144	5′ GTAAGCCAGGTTAGTTTCTA (H)
HSP 1	14 061-14 080	5′ CTTTGTCCGTTACTATTACT (H)
HSP 2	13 890-13 909	5′ ACTAGCTAGAATCAAACTTT (L)
HSP 3	14 171-14 190	5′ TCAAAGCAGTCTCCCCGGCG (H)
HSP 4	14 250-14 269	5′ ATTTAAATGTGGAAAAATAC (H)
HSP 5	13 921-13 940	5′ TCACTGCACTTCTAACCTAT (L)

^*

Numbered according to Valverde et al.(1994a). Accession number NC 001620.

H and L denote heavy and light chain, respectively.