. | . | % of activity in compartment . | . | . | ||
---|---|---|---|---|---|---|
Treatment/tissue . | Enzyme activity (μmol min-1 g-1 wet tissue) . | Soluble . | Mitochondrial . | Debris . | ||
Confined | ||||||
Glutamine synthetase | ||||||
Liver | 15.55±1.92 | 64.8±6.8 | 13.1±3.0 | 22.1±5.2 | ||
Gill | 1.93±0.61 | 81.8±3.3 | 3.0±0.8 | 15.2±3.6 | ||
Brain | 37.52±1.51 | 52.7±0.7 | 29.6±1.7 | 17.7±1.4 | ||
Intestine | 3.25 | 58.7 | 9.9 | 31.5 | ||
Stomach | 4.17 | 66.8 | 5.2 | 28.0 | ||
Muscle | 0.13 | 73.8 | 15.2 | 11.0 | ||
Kidney | 1.96 | 42.4 | 17.5 | 40.1 | ||
Glutamate dehydrogenase (mitochondrial) | ||||||
Liver | 11.49±0.88 | 8.2±0.6 | 53.0±6.2 | 38.9±6.5 | ||
Gill | 5.88±0.98 | 12.0±4.1 | 53.9±6.4 | 34.1±7.4 | ||
Brain | 1.88±0.28 | 13.8±1.9 | 54.5±5.6 | 31.8±6.3 | ||
Intestine | 3.95 | 12.6 | 57.4 | 30.0 | ||
Stomach | 4.22 | 20.2 | 36.1 | 43.6 | ||
Muscle | 0.97 | 29.9 | 23.4 | 46.7 | ||
Kidney | 7.27 | 11.7 | 52.2 | 36.1 | ||
Lactate dehydrogenase (soluble) | ||||||
Liver | 6.87±2.07 | 69.8±4.3 | 11.9±3.6 | 18.3±2.3 | ||
Gill | 32.45±6.97 | 69.3±3.6 | 7.8±4.0 | 23.0±1.9 | ||
Brain | 47.76±9.02 | 46.9±2.4 | 30.4±1.3 | 22.7±1.6 | ||
Intestine | 19.95 | 59.8 | 8.7 | 31.5 | ||
Stomach | 20.86 | 69.1 | 3.6 | 27.3 | ||
Muscle | 145.29 | 68.1 | 1.0 | 30.9 | ||
Kidney | 16.95 | 73.5 | 4.6 | 21.9 | ||
Unconfined | ||||||
Glutamine synthetase | ||||||
Liver | 4.41 | 36.0 | 44.1 | 19.9 | ||
Gill | 2.57 | 82.9 | 5.2 | 12.0 | ||
Brain | 32.46 | 54.2 | 30.5 | 15.3 | ||
Intestine | 2.95 | 66.1 | 14.7 | 19.2 | ||
Stomach | 5.48 | 67.3 | 5.1 | 27.6 | ||
Muscle | 0.17 | 58.7 | 8.6 | 32.8 | ||
Kidney | 1.47 | 36.5 | 25.2 | 38.4 |
. | . | % of activity in compartment . | . | . | ||
---|---|---|---|---|---|---|
Treatment/tissue . | Enzyme activity (μmol min-1 g-1 wet tissue) . | Soluble . | Mitochondrial . | Debris . | ||
Confined | ||||||
Glutamine synthetase | ||||||
Liver | 15.55±1.92 | 64.8±6.8 | 13.1±3.0 | 22.1±5.2 | ||
Gill | 1.93±0.61 | 81.8±3.3 | 3.0±0.8 | 15.2±3.6 | ||
Brain | 37.52±1.51 | 52.7±0.7 | 29.6±1.7 | 17.7±1.4 | ||
Intestine | 3.25 | 58.7 | 9.9 | 31.5 | ||
Stomach | 4.17 | 66.8 | 5.2 | 28.0 | ||
Muscle | 0.13 | 73.8 | 15.2 | 11.0 | ||
Kidney | 1.96 | 42.4 | 17.5 | 40.1 | ||
Glutamate dehydrogenase (mitochondrial) | ||||||
Liver | 11.49±0.88 | 8.2±0.6 | 53.0±6.2 | 38.9±6.5 | ||
Gill | 5.88±0.98 | 12.0±4.1 | 53.9±6.4 | 34.1±7.4 | ||
Brain | 1.88±0.28 | 13.8±1.9 | 54.5±5.6 | 31.8±6.3 | ||
Intestine | 3.95 | 12.6 | 57.4 | 30.0 | ||
Stomach | 4.22 | 20.2 | 36.1 | 43.6 | ||
Muscle | 0.97 | 29.9 | 23.4 | 46.7 | ||
Kidney | 7.27 | 11.7 | 52.2 | 36.1 | ||
Lactate dehydrogenase (soluble) | ||||||
Liver | 6.87±2.07 | 69.8±4.3 | 11.9±3.6 | 18.3±2.3 | ||
Gill | 32.45±6.97 | 69.3±3.6 | 7.8±4.0 | 23.0±1.9 | ||
Brain | 47.76±9.02 | 46.9±2.4 | 30.4±1.3 | 22.7±1.6 | ||
Intestine | 19.95 | 59.8 | 8.7 | 31.5 | ||
Stomach | 20.86 | 69.1 | 3.6 | 27.3 | ||
Muscle | 145.29 | 68.1 | 1.0 | 30.9 | ||
Kidney | 16.95 | 73.5 | 4.6 | 21.9 | ||
Unconfined | ||||||
Glutamine synthetase | ||||||
Liver | 4.41 | 36.0 | 44.1 | 19.9 | ||
Gill | 2.57 | 82.9 | 5.2 | 12.0 | ||
Brain | 32.46 | 54.2 | 30.5 | 15.3 | ||
Intestine | 2.95 | 66.1 | 14.7 | 19.2 | ||
Stomach | 5.48 | 67.3 | 5.1 | 27.6 | ||
Muscle | 0.17 | 58.7 | 8.6 | 32.8 | ||
Kidney | 1.47 | 36.5 | 25.2 | 38.4 |
Values are means ± 1 S.E.M. N=4 for liver, gill and brain of confined fish; N=1 for other tissues and unconfined treatment.
All samples contained the pooled tissues of 3-5 fish.