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Table 2.

Primers used to amplify the glutamine synthetase gene fragments

PCR product(s)Forward primerReverse primer
CA2-1 (a—d) GLUL*e3f1 GLUL*e6r1 
CA2-2 (a, b) GLUL*e5f1 GLUL*e6r1 
CA3-21 GLUL-Onmy*e4f1 T7 
CA3-22 GLUL-Onmy*e4f1 M13 
CA3-23 GLUL-Onmy*e5r1 T7 
CA3-25 GLUL-Onmy*e4f2 T7 
CA3-26 GLUL-Onmy*e5r2 M13 
CA0407 GLUL-Onmy*e7f1 M13 
CA0504 GLUL-Onmy*e6f1 pBKCMV*r1 
CA0508 GLUL-Onmy*e6f1 pBKCMV*f1 
CA0509 GLUL-Onmy*e41 GLUL-Onmy*3UR1 
RTGS3, 4 GS501 GS301 
RTGS3b GS031i 3′RACE UAP 
RTGS5 RTGS51 3′RACE UAP 
PCR product(s)Forward primerReverse primer
CA2-1 (a—d) GLUL*e3f1 GLUL*e6r1 
CA2-2 (a, b) GLUL*e5f1 GLUL*e6r1 
CA3-21 GLUL-Onmy*e4f1 T7 
CA3-22 GLUL-Onmy*e4f1 M13 
CA3-23 GLUL-Onmy*e5r1 T7 
CA3-25 GLUL-Onmy*e4f2 T7 
CA3-26 GLUL-Onmy*e5r2 M13 
CA0407 GLUL-Onmy*e7f1 M13 
CA0504 GLUL-Onmy*e6f1 pBKCMV*r1 
CA0508 GLUL-Onmy*e6f1 pBKCMV*f1 
CA0509 GLUL-Onmy*e41 GLUL-Onmy*3UR1 
RTGS3, 4 GS501 GS301 
RTGS3b GS031i 3′RACE UAP 
RTGS5 RTGS51 3′RACE UAP 
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