Skip to Main Content
Table 1.

PCR primers used to amplify glutamine synthetase genes

PrimerDNA sequence (5′-3′)Location1Orientation
GLUL*e3f1 GAR GGC TCC AAC AGY GAC ATG TA 78 
GLUL*e4r1 TAC TCC TGY TCC ATK CCA AAC CA 132 
GLUL*e5f1 GTG GAG GCN CAT TAY AGA GCC TG 185 
GLUL*e6r1 TTG GTG CTR AAG TTT GTR TGG CA 254 
GLUL-Onmy*e4f1 AAG AAG ATC ATG GAG ATG GTA 126 
GLUL-Onmy*e4f2 GGT TTG GCA TGG AGC AAG AG 139 
GLUL-Onmy*e5r1 TAG TGG GCT TCT ACG ATG TC 176 
GLUL-Onmy*e5r2 CAT TGG TGC CAC AGA TCA TG 190 
GLUL-Onmy*e6f1 GGT CCT TGT GAA GGC ATC AC 215 
GLUL-Onmy*e6f2 CCA TAC AAA CTT CAG CAC CAA G 261 
GLUL-Onmy*e7f1 CAC CAC GAA ACR TCC AAC AT 311 
GLUL-Onmy*e7f2 AGG CTG GGG AGG AGG CAT A 284 
GLUL-Onmy*e7f2 AGG CTG GGG AGG AGG CAT A 284 
GLUL-Onmy*3UR1 TGA GAT GTA GCG CAA TAG GAC 38 bp2 
GLUL-Onmy*3UR3 CAG TGG ATC TCG TTG GGT CTA 374 
GS301 CAG CCA GCA CCG TTC CAG TT 248 
GS501 GAG GCA TCC AAC AGC GAC ATG T 78 
GS031I AAC TGG AAC GGT GCT GGC TG 254 
RTGS51 TGG TTC GGC ATG GAR CAR GAR TA 139 
pBKCMV*r1 GCG AAT TGG GTA CAC TTA CCT G na na 
pBKCMV*f1 CTA AAG GGA ACA AAA GCT GGA GC na na 
M13r TCA CAC AGG AAA CAG CTA TGA CC na na 
T7 TAA TAC GAC TCA CTA TAG GGC na na 
PrimerDNA sequence (5′-3′)Location1Orientation
GLUL*e3f1 GAR GGC TCC AAC AGY GAC ATG TA 78 
GLUL*e4r1 TAC TCC TGY TCC ATK CCA AAC CA 132 
GLUL*e5f1 GTG GAG GCN CAT TAY AGA GCC TG 185 
GLUL*e6r1 TTG GTG CTR AAG TTT GTR TGG CA 254 
GLUL-Onmy*e4f1 AAG AAG ATC ATG GAG ATG GTA 126 
GLUL-Onmy*e4f2 GGT TTG GCA TGG AGC AAG AG 139 
GLUL-Onmy*e5r1 TAG TGG GCT TCT ACG ATG TC 176 
GLUL-Onmy*e5r2 CAT TGG TGC CAC AGA TCA TG 190 
GLUL-Onmy*e6f1 GGT CCT TGT GAA GGC ATC AC 215 
GLUL-Onmy*e6f2 CCA TAC AAA CTT CAG CAC CAA G 261 
GLUL-Onmy*e7f1 CAC CAC GAA ACR TCC AAC AT 311 
GLUL-Onmy*e7f2 AGG CTG GGG AGG AGG CAT A 284 
GLUL-Onmy*e7f2 AGG CTG GGG AGG AGG CAT A 284 
GLUL-Onmy*3UR1 TGA GAT GTA GCG CAA TAG GAC 38 bp2 
GLUL-Onmy*3UR3 CAG TGG ATC TCG TTG GGT CTA 374 
GS301 CAG CCA GCA CCG TTC CAG TT 248 
GS501 GAG GCA TCC AAC AGC GAC ATG T 78 
GS031I AAC TGG AAC GGT GCT GGC TG 254 
RTGS51 TGG TTC GGC ATG GAR CAR GAR TA 139 
pBKCMV*r1 GCG AAT TGG GTA CAC TTA CCT G na na 
pBKCMV*f1 CTA AAG GGA ACA AAA GCT GGA GC na na 
M13r TCA CAC AGG AAA CAG CTA TGA CC na na 
T7 TAA TAC GAC TCA CTA TAG GGC na na 

na, not applicable.

1

Location of the 3′ end of primer relative to amino acid position(Fig. 2).

2

Position given in base pairs (bp) from stop codon relative to 3′ end of primer.

Close Modal

or Create an Account

Close Modal
Close Modal