PCR primers used to amplify glutamine synthetase genes
| Primer . | DNA sequence (5′-3′) . | Location1 . | Orientation . |
|---|---|---|---|
| GLUL*e3f1 | GAR GGC TCC AAC AGY GAC ATG TA | 78 | F |
| GLUL*e4r1 | TAC TCC TGY TCC ATK CCA AAC CA | 132 | R |
| GLUL*e5f1 | GTG GAG GCN CAT TAY AGA GCC TG | 185 | F |
| GLUL*e6r1 | TTG GTG CTR AAG TTT GTR TGG CA | 254 | R |
| GLUL-Onmy*e4f1 | AAG AAG ATC ATG GAG ATG GTA | 126 | F |
| GLUL-Onmy*e4f2 | GGT TTG GCA TGG AGC AAG AG | 139 | F |
| GLUL-Onmy*e5r1 | TAG TGG GCT TCT ACG ATG TC | 176 | R |
| GLUL-Onmy*e5r2 | CAT TGG TGC CAC AGA TCA TG | 190 | R |
| GLUL-Onmy*e6f1 | GGT CCT TGT GAA GGC ATC AC | 215 | F |
| GLUL-Onmy*e6f2 | CCA TAC AAA CTT CAG CAC CAA G | 261 | F |
| GLUL-Onmy*e7f1 | CAC CAC GAA ACR TCC AAC AT | 311 | F |
| GLUL-Onmy*e7f2 | AGG CTG GGG AGG AGG CAT A | 284 | F |
| GLUL-Onmy*e7f2 | AGG CTG GGG AGG AGG CAT A | 284 | F |
| GLUL-Onmy*3UR1 | TGA GAT GTA GCG CAA TAG GAC | 38 bp2 | R |
| GLUL-Onmy*3UR3 | CAG TGG ATC TCG TTG GGT CTA | 374 | R |
| GS301 | CAG CCA GCA CCG TTC CAG TT | 248 | R |
| GS501 | GAG GCA TCC AAC AGC GAC ATG T | 78 | F |
| GS031I | AAC TGG AAC GGT GCT GGC TG | 254 | F |
| RTGS51 | TGG TTC GGC ATG GAR CAR GAR TA | 139 | F |
| pBKCMV*r1 | GCG AAT TGG GTA CAC TTA CCT G | na | na |
| pBKCMV*f1 | CTA AAG GGA ACA AAA GCT GGA GC | na | na |
| M13r | TCA CAC AGG AAA CAG CTA TGA CC | na | na |
| T7 | TAA TAC GAC TCA CTA TAG GGC | na | na |
| Primer . | DNA sequence (5′-3′) . | Location1 . | Orientation . |
|---|---|---|---|
| GLUL*e3f1 | GAR GGC TCC AAC AGY GAC ATG TA | 78 | F |
| GLUL*e4r1 | TAC TCC TGY TCC ATK CCA AAC CA | 132 | R |
| GLUL*e5f1 | GTG GAG GCN CAT TAY AGA GCC TG | 185 | F |
| GLUL*e6r1 | TTG GTG CTR AAG TTT GTR TGG CA | 254 | R |
| GLUL-Onmy*e4f1 | AAG AAG ATC ATG GAG ATG GTA | 126 | F |
| GLUL-Onmy*e4f2 | GGT TTG GCA TGG AGC AAG AG | 139 | F |
| GLUL-Onmy*e5r1 | TAG TGG GCT TCT ACG ATG TC | 176 | R |
| GLUL-Onmy*e5r2 | CAT TGG TGC CAC AGA TCA TG | 190 | R |
| GLUL-Onmy*e6f1 | GGT CCT TGT GAA GGC ATC AC | 215 | F |
| GLUL-Onmy*e6f2 | CCA TAC AAA CTT CAG CAC CAA G | 261 | F |
| GLUL-Onmy*e7f1 | CAC CAC GAA ACR TCC AAC AT | 311 | F |
| GLUL-Onmy*e7f2 | AGG CTG GGG AGG AGG CAT A | 284 | F |
| GLUL-Onmy*e7f2 | AGG CTG GGG AGG AGG CAT A | 284 | F |
| GLUL-Onmy*3UR1 | TGA GAT GTA GCG CAA TAG GAC | 38 bp2 | R |
| GLUL-Onmy*3UR3 | CAG TGG ATC TCG TTG GGT CTA | 374 | R |
| GS301 | CAG CCA GCA CCG TTC CAG TT | 248 | R |
| GS501 | GAG GCA TCC AAC AGC GAC ATG T | 78 | F |
| GS031I | AAC TGG AAC GGT GCT GGC TG | 254 | F |
| RTGS51 | TGG TTC GGC ATG GAR CAR GAR TA | 139 | F |
| pBKCMV*r1 | GCG AAT TGG GTA CAC TTA CCT G | na | na |
| pBKCMV*f1 | CTA AAG GGA ACA AAA GCT GGA GC | na | na |
| M13r | TCA CAC AGG AAA CAG CTA TGA CC | na | na |
| T7 | TAA TAC GAC TCA CTA TAG GGC | na | na |