The effect of 1 mmol l-1 sodium nitroprusside (SNP) on Na+ and K+ content and inulin space in kidney and gill tissue from brown trout
. | Na+ content (nmol mg-1 wet mass) . | K+ content (nmol mg-1 wet mass) . | Inulin space (μl mg-1 wet mass) . | cGMP concentration (fmol mg-1 protein) . |
---|---|---|---|---|
Kidney tissue | ||||
Control | 65.8±1.1 | 78.6±0.6 | 0.142±0.009 | 53±4 |
SNP | 73.3±1.1* | 73.6±0.8* | 0.139±0.004 | 469±62* |
Gill tissue | ||||
Control | 89.1±1.1 | 69.9±0.8 | 0.124±0.004 | 230±27 |
SNP | 89.8±1.3 | 68.9±1.2 | 0.120±0.005 | 365±31* |
. | Na+ content (nmol mg-1 wet mass) . | K+ content (nmol mg-1 wet mass) . | Inulin space (μl mg-1 wet mass) . | cGMP concentration (fmol mg-1 protein) . |
---|---|---|---|---|
Kidney tissue | ||||
Control | 65.8±1.1 | 78.6±0.6 | 0.142±0.009 | 53±4 |
SNP | 73.3±1.1* | 73.6±0.8* | 0.139±0.004 | 469±62* |
Gill tissue | ||||
Control | 89.1±1.1 | 69.9±0.8 | 0.124±0.004 | 230±27 |
SNP | 89.8±1.3 | 68.9±1.2 | 0.120±0.005 | 365±31* |
The tissues were incubated under standard conditions for 2×30 min. Cyclic GMP (cGMP) concentrations were determined in both tissues following 10 min incubation with or without 1 mmol l-1 SNP.
Significant effect of treatment (P<0.05). Values are means± S.E.M. (N=6).