Intra-and extracellular acid-base variables of isolated hepatocytes prepared from P. brachycephalum
| pHe . | pHi . | PCO2 (kPa) . | [HCO3−]i (mmol l−1) . | [HCO3−]e (mmol l−1) . |
|---|---|---|---|---|
| 7.90 | 7.65±0.08 | 1.02 | 10.45 | 48.95 |
| 7.90 | 7.58±0.05 | 0.03 | 0.39 | 1.54 |
| 7.20 | 7.23±0.05 | 1.02 | 4.12 | 8.68 |
| 7.20 | 7.17±0.07 | 0.03 | 0.15 | 0.27 |
| 6.50 | 6.98±0.05 | 1.02 | 2.61 | 1.68 |
| 6.50 | 6.98±0.07 | 0.03 | 0.08 | 0.05 |
| pHe . | pHi . | PCO2 (kPa) . | [HCO3−]i (mmol l−1) . | [HCO3−]e (mmol l−1) . |
|---|---|---|---|---|
| 7.90 | 7.65±0.08 | 1.02 | 10.45 | 48.95 |
| 7.90 | 7.58±0.05 | 0.03 | 0.39 | 1.54 |
| 7.20 | 7.23±0.05 | 1.02 | 4.12 | 8.68 |
| 7.20 | 7.17±0.07 | 0.03 | 0.15 | 0.27 |
| 6.50 | 6.98±0.05 | 1.02 | 2.61 | 1.68 |
| 6.50 | 6.98±0.07 | 0.03 | 0.08 | 0.05 |
pHi values are means ± s.d., N=5-6.
Intracellular CO2 partial pressure was assumed to equal medium PCO2.
Apparent mean intracellular bicarbonate concentrations were calculated from mean pHi values by use of the Henderson-Hasselbach equation (see Materials and methods).