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Table 4.

Na+/K+-ATPase α3-mRNA expression in gills of rainbow trout before (Pre) and 8 h, 5 days and 15 days following abrupt transfer to freshwater, 40% seawater and 80% seawater

Expression of Na+/K+-ATPase α3 (relative to EF-1α)
TreatmentPre8 h5 days15 days
Freshwater 1.00±0.15 1.44±0.22 1.67±0.67 0.85±0.11 
40% seawater 1.00±0.07 1.10±0.37 1.67±0.77 0.80±0.10 
80% seawater 1.00±0.07 1.10±0.28 1.07±0.17 1.24±0.38 
Expression of Na+/K+-ATPase α3 (relative to EF-1α)
TreatmentPre8 h5 days15 days
Freshwater 1.00±0.15 1.44±0.22 1.67±0.67 0.85±0.11 
40% seawater 1.00±0.07 1.10±0.37 1.67±0.77 0.80±0.10 
80% seawater 1.00±0.07 1.10±0.28 1.07±0.17 1.24±0.38 

Na+/K+-ATPase α3 mRNA expression is relative to EF-1α expression, and all data following the transfer are relative to the pre-transfer freshwater gill samples.

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