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Table 6.

Concentration (10⁵ cells ml⁻¹) of haemocyte sub-populations in C. gigas hemolymph samples during DCFH-DA analysis

	18°C					30°C
	C. calcitrans	T. suecica	T-Iso	C. calcitrans	T. suecica	T-Iso
Control condition
Granulocytes	1.0±0.05^b,A	0.6±0.3^a,b,A	0.5±0.2^a,A	1.1±0.04^b,A	0.6±0.2^a,A	0.5±0.2^a,A
Hyalinocytes	2.0±0.2^a,A	1.4±0.2^a,A	1.5±0.2^a,A	2.3±0.2^b,A	1.5±0.3^a,A	1.6±0.2^a,A
Small agranular cells	1.1±0.1^a,A	0.8±0.2^a,A	0.9±0.3^a,A	1.1±0.1^a,A	0.9±0.3^a,A	0.9±0.2^a,A
With PMA
Granulocytes	0.4±0.1^a,B	0.5±0.2^a,A	0.3±0.1^a,A	0.5±0.1^a,B	0.4±0.1^a,A	0.3±0.1^a,A
Hyalinocytes	1.6±0.3^a,A	1.3±0.4^a,A	1.2±0.1^a,A	1.8±0.4^a,A	1.3±0.2^a,A	1.3±0.1^a,A
Small agranular cells	0.9±0.1^a,A	0.9±0.3^a,A	0.8±0.2^a,A	0.8±0.1^a,A	0.8±0.2^a,A	0.7±0.2^a,A

	18°C					30°C
	C. calcitrans	T. suecica	T-Iso	C. calcitrans	T. suecica	T-Iso
Control condition
Granulocytes	1.0±0.05^b,A	0.6±0.3^a,b,A	0.5±0.2^a,A	1.1±0.04^b,A	0.6±0.2^a,A	0.5±0.2^a,A
Hyalinocytes	2.0±0.2^a,A	1.4±0.2^a,A	1.5±0.2^a,A	2.3±0.2^b,A	1.5±0.3^a,A	1.6±0.2^a,A
Small agranular cells	1.1±0.1^a,A	0.8±0.2^a,A	0.9±0.3^a,A	1.1±0.1^a,A	0.9±0.3^a,A	0.9±0.2^a,A
With PMA
Granulocytes	0.4±0.1^a,B	0.5±0.2^a,A	0.3±0.1^a,A	0.5±0.1^a,B	0.4±0.1^a,A	0.3±0.1^a,A
Hyalinocytes	1.6±0.3^a,A	1.3±0.4^a,A	1.2±0.1^a,A	1.8±0.4^a,A	1.3±0.2^a,A	1.3±0.1^a,A
Small agranular cells	0.9±0.1^a,A	0.9±0.3^a,A	0.8±0.2^a,A	0.8±0.1^a,A	0.8±0.2^a,A	0.7±0.2^a,A

DCFH-DA, 2′7′ -dichlorofluorescein diacetate; PMA, phorbol myristate acetate.

Different lower-case letters indicate significant difference between dietary treatments. Different upper-case letters indicate a significant effect of the analysis conditions (control vs PMA; mean ± s.d., N=3, ANOVA P<0.01 for dietary treatment; t-test P<0.05 for the PMA effect).

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