The Company of Biologists

Table 4.

Total haemocyte count (THC), viability rate of haemocytes (measured with propidium iodide) and adhesion rate of oyster and clam haemocytes after two hours of incubation at 18°C and 30°C

		T=8 weeks
	Initial	C. calcitrans	T. suecica	T-Iso
THC (10⁵ cells ml⁻¹)
Oysters	6,4±2.2	5,8±1.0^a	3,7±1.1^a	3,7±1.0^a
Clams	5,9±1.2	14±2.5^a,*	9,6±5.5^a,b	8±1.2^b
Viability (% of live cells)
Oysters	97.2±1.5	95.9±0.8^a	96.7±1.3^a	96.1±0.9^a
Clams	97±0.8	96.4±0.5^a	97.4±0.8^a	95.2±1.5^a
Adhesion (% of adhered cells)
18°C
Oysters	ND	91.8±0.5^a	91.4±2.7^a	90.6±1.1^a
Clams	ND	98.9±0.9^a	98.5±1.2^a	99.0±0.4^a
30°C
Oysters	ND	89.4±0.4^a	89.6±0.9^a	88.5±1.9^a
Clams	ND	98.0±0.7^a	96.7±0.7^a	96.7±1.1^a

		T=8 weeks
	Initial	C. calcitrans	T. suecica	T-Iso
THC (10⁵ cells ml⁻¹)
Oysters	6,4±2.2	5,8±1.0^a	3,7±1.1^a	3,7±1.0^a
Clams	5,9±1.2	14±2.5^a,*	9,6±5.5^a,b	8±1.2^b
Viability (% of live cells)
Oysters	97.2±1.5	95.9±0.8^a	96.7±1.3^a	96.1±0.9^a
Clams	97±0.8	96.4±0.5^a	97.4±0.8^a	95.2±1.5^a
Adhesion (% of adhered cells)
18°C
Oysters	ND	91.8±0.5^a	91.4±2.7^a	90.6±1.1^a
Clams	ND	98.9±0.9^a	98.5±1.2^a	99.0±0.4^a
30°C
Oysters	ND	89.4±0.4^a	89.6±0.9^a	88.5±1.9^a
Clams	ND	98.0±0.7^a	96.7±0.7^a	96.7±1.1^a

Different lower-case letters indicate significant difference between dietary treatments (mean ± s.d.; N=3; ANOVA P<0.05 for THC and viability and P>0.05 for adhesion). ND, not determined. Asterisks indicate significant difference between a dietary treatment and initial at P<0.05.

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