Effect of forskolin (FSK), SQ22,536 and MDL-12,330A on the kinetic parameters (LT and TLmax) of KCl-induced luminescence on O. californica and O. aranea dissociated photocytes
| . | LT (s) . | TLmax (s) . |
|---|---|---|
| O. californica (N=10) | ||
| 50 mmol l−1 KCl (control for FSK) | 8.30±3.43 | 11.15±3.43 |
| 10−4 mol 1−1 FSK | 0.01* | 1.25±0.33* |
| 200 mmol l−1 KCl (control for SQ) | 0.01 | 0.55±0.15 |
| 10−5 mol 1−1 SQ22,536 | 0.01 | 3.20±0.86* |
| 200 mmol l−1 KCl (control for MDL) | 0.01 | 0.50±0.16 |
| 10−5 mol 1−1 MDL-12,330A | 0.01 | 3.72±0.73* |
| O. aranea (N=10) | ||
| 200 mmol l−1 KCl (control for MDL) | 0.28±0.22 | 0.61±0.11 |
| 10−5 mol 1−1 MDL-12,330A | 0.20±0.08 | 2.00±0.37* |
| 200 mmol l−1 KCl (control for SQ) | 0.01 | 0.10±0.06 |
| 10−5 mol 1−1 SQ22,536 | 0.05±0.04 | 2.60±0.71* |
| . | LT (s) . | TLmax (s) . |
|---|---|---|
| O. californica (N=10) | ||
| 50 mmol l−1 KCl (control for FSK) | 8.30±3.43 | 11.15±3.43 |
| 10−4 mol 1−1 FSK | 0.01* | 1.25±0.33* |
| 200 mmol l−1 KCl (control for SQ) | 0.01 | 0.55±0.15 |
| 10−5 mol 1−1 SQ22,536 | 0.01 | 3.20±0.86* |
| 200 mmol l−1 KCl (control for MDL) | 0.01 | 0.50±0.16 |
| 10−5 mol 1−1 MDL-12,330A | 0.01 | 3.72±0.73* |
| O. aranea (N=10) | ||
| 200 mmol l−1 KCl (control for MDL) | 0.28±0.22 | 0.61±0.11 |
| 10−5 mol 1−1 MDL-12,330A | 0.20±0.08 | 2.00±0.37* |
| 200 mmol l−1 KCl (control for SQ) | 0.01 | 0.10±0.06 |
| 10−5 mol 1−1 SQ22,536 | 0.05±0.04 | 2.60±0.71* |
The results for A. filiformis are not shown since no significant effect of any of the drugs was observed on the kinetic parameters of light emission in this species. Means ± s.e.m. are expressed in seconds; values without s.e.m. are due to the fact that the same LT was observed for all repetitions. *P<0.01; N, number of stimulated aliquot parts.