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Table 6.

Changes in the units of activities per g tissueof glutamine synthetase(GS), carbamoylphosphate synthetase (CPS), ornithine transcarbamoylase (OTC),arginosuccinate synthetase + lyase (ASS+L) and arginase in the stomach ofH. signifer after progressive increase in salinity from 0.7‰ to 10‰ or 20‰

Enzyme activity (μmol min−1 g−1 wet mass)
EnzymeSubstrate and effector0.7‰ (control)10‰20‰
GS 6.03±0.86 10.5±2.90 6.84±0.68 
OTC 8.40±0.86 6.71±1.92 5.25±0.72 
ASS+L 0.42±0.09 0.45±0.11 0.46±0.04 
Arginase 5.57±2.22 15.1±3.26 5.40±0.30 
CPS Glutamine 0.03±0.01 0.01±0.005 0.15±0.07*, 
 Glutamine + AGA 0.10±0.05 0.03±0.01 0.45±0.18*, 
 Glutamine + AGA + UTP 0.09±0.04 0.03±0.01 0.41±0.16*, 
Enzyme activity (μmol min−1 g−1 wet mass)
EnzymeSubstrate and effector0.7‰ (control)10‰20‰
GS 6.03±0.86 10.5±2.90 6.84±0.68 
OTC 8.40±0.86 6.71±1.92 5.25±0.72 
ASS+L 0.42±0.09 0.45±0.11 0.46±0.04 
Arginase 5.57±2.22 15.1±3.26 5.40±0.30 
CPS Glutamine 0.03±0.01 0.01±0.005 0.15±0.07*, 
 Glutamine + AGA 0.10±0.05 0.03±0.01 0.45±0.18*, 
 Glutamine + AGA + UTP 0.09±0.04 0.03±0.01 0.41±0.16*, 

AGA, N-acetyl-l-glutamate; UTP, uridine triphosphate.

Results represent means ± s.e.m. (N=4).

*

Significantly different from 0.7‰ control condition at P<0.05.

Significantly different from 10‰ at P<0.05.

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