Composition of experimental solutions(Nicolson, 1992)
| . | . | Composition (mmol l-1) . | . | |
|---|---|---|---|---|
| . | Control . | A (K+ free) . | B (low Na+) . | |
| NaCl | 90 | 140 | - | |
| KCl | 50 | - | 140 | |
| MgCl2 | 5 | 5 | 5 | |
| CaCl2 | 2 | 2 | 2 | |
| NaHCO3 | 6 | 6 | 6 | |
| NaH2PO4 | 4 | 4 | 4 | |
| Glycine | 10 | 10 | 10 | |
| Proline | 10 | 10 | 10 | |
| Serine | 10 | 10 | 10 | |
| Histidine | 10 | 10 | 10 | |
| Glutamine | 10 | 10 | 10 | |
| Glucose | 50 | 50 | 50 | |
| . | . | Composition (mmol l-1) . | . | |
|---|---|---|---|---|
| . | Control . | A (K+ free) . | B (low Na+) . | |
| NaCl | 90 | 140 | - | |
| KCl | 50 | - | 140 | |
| MgCl2 | 5 | 5 | 5 | |
| CaCl2 | 2 | 2 | 2 | |
| NaHCO3 | 6 | 6 | 6 | |
| NaH2PO4 | 4 | 4 | 4 | |
| Glycine | 10 | 10 | 10 | |
| Proline | 10 | 10 | 10 | |
| Serine | 10 | 10 | 10 | |
| Histidine | 10 | 10 | 10 | |
| Glutamine | 10 | 10 | 10 | |
| Glucose | 50 | 50 | 50 | |
pH was adjusted to 7.00 by adding 1 mol l-1 HCl. Osmolality of all solutions was 390 mosmol kg-1. Different K+concentrations were obtained by mixing solutions A and B.