. | Enzyme activity (μmol substrate h-1) . | . | . | . | |||
---|---|---|---|---|---|---|---|
Fraction . | Alkaline phosphatase . | Na+,K+-ATPase . | Cytochrome c oxidase . | Glucose-6-phosphatase . | |||
Dorsal | |||||||
Homogenate | 221.21±54.09 | 36.58±5.73 | 111.64±16.86 | 11.39±3.62 | |||
BBMV | 43.48±13.84 | 0.11±0.02 | 0.10±0.02 | 0.03±0.02 | |||
Recovery(%)* | 20.52±6.39 | 0.34±0.04 | 0.11±0.03 | 0.15±0.08 | |||
Contamination (%)† | 0.47±0.20 | 0.49±0.23 | 0.03±0.02 | ||||
Ventral | |||||||
Homogenate | 273.79±73.92 | 35.06±8.68 | 119.40±15.48 | 12.00±4.45 | |||
BBMV | 52.77±19.87 | 0.13±0.02 | 0.11±0.03 | 0.11±0.10 | |||
Recovery (%) | 23.94±8.70 | 0.42±0.07 | 0.09±0.02 | 0.44±0.20 | |||
Contamination (%) | 0.38±0.09 | 0.37±0.11 | 0.09±0.07 |
. | Enzyme activity (μmol substrate h-1) . | . | . | . | |||
---|---|---|---|---|---|---|---|
Fraction . | Alkaline phosphatase . | Na+,K+-ATPase . | Cytochrome c oxidase . | Glucose-6-phosphatase . | |||
Dorsal | |||||||
Homogenate | 221.21±54.09 | 36.58±5.73 | 111.64±16.86 | 11.39±3.62 | |||
BBMV | 43.48±13.84 | 0.11±0.02 | 0.10±0.02 | 0.03±0.02 | |||
Recovery(%)* | 20.52±6.39 | 0.34±0.04 | 0.11±0.03 | 0.15±0.08 | |||
Contamination (%)† | 0.47±0.20 | 0.49±0.23 | 0.03±0.02 | ||||
Ventral | |||||||
Homogenate | 273.79±73.92 | 35.06±8.68 | 119.40±15.48 | 12.00±4.45 | |||
BBMV | 52.77±19.87 | 0.13±0.02 | 0.11±0.03 | 0.11±0.10 | |||
Recovery (%) | 23.94±8.70 | 0.42±0.07 | 0.09±0.02 | 0.44±0.20 | |||
Contamination (%) | 0.38±0.09 | 0.37±0.11 | 0.09±0.07 |
BBMV, brush border membrane vesicles
Values are means ± s.e.m. (N=6)
Recovery was calculated as the percentage of the total activity in the BBM fraction relative to the total activity in the initial tissue homogenate
Contamination was calculated as the percentage of the total activity in the BBM fraction relative to the total activity of alkaline phosphatase in the BBM fraction