. | Enzyme specific activity (μmol substrate h-1 mg-1protein) . | . | . | . | |||
---|---|---|---|---|---|---|---|
Fraction . | Alkaline phosphatase . | Na+,K+-ATPase . | Cytochrome c oxidase . | Glucose-6-phosphatase . | |||
Dorsal | |||||||
Homogenate | 3.17±0.68 | 0.53±0.04 | 1.66±0.20 | 0.14±0.04 | |||
BBMV | 173.56±42.75 | 0.58±0.14 | 0.58±0.19 | 0.08±0.04 | |||
Magnitude of purification (-fold) | 63.44±16.39 | 1.12±0.20 | 0.35±0.10 | 0.44±0.19 | |||
Ventral | |||||||
Homogenate | 3.84±1.13 | 0.48±0.06 | 1.77±0.20 | 0.14±0.03 | |||
BBMV | 162.99±52.12 | 0.48±0.08 | 0.43±0.10 | 0.19±0.14 | |||
Magnitude of purification (-fold) | 61.23±19.39 | 1.12±0.28 | 0.23±0.04 | 1.06±0.27 |
. | Enzyme specific activity (μmol substrate h-1 mg-1protein) . | . | . | . | |||
---|---|---|---|---|---|---|---|
Fraction . | Alkaline phosphatase . | Na+,K+-ATPase . | Cytochrome c oxidase . | Glucose-6-phosphatase . | |||
Dorsal | |||||||
Homogenate | 3.17±0.68 | 0.53±0.04 | 1.66±0.20 | 0.14±0.04 | |||
BBMV | 173.56±42.75 | 0.58±0.14 | 0.58±0.19 | 0.08±0.04 | |||
Magnitude of purification (-fold) | 63.44±16.39 | 1.12±0.20 | 0.35±0.10 | 0.44±0.19 | |||
Ventral | |||||||
Homogenate | 3.84±1.13 | 0.48±0.06 | 1.77±0.20 | 0.14±0.03 | |||
BBMV | 162.99±52.12 | 0.48±0.08 | 0.43±0.10 | 0.19±0.14 | |||
Magnitude of purification (-fold) | 61.23±19.39 | 1.12±0.28 | 0.23±0.04 | 1.06±0.27 |
BBMV, brush border membrane vesicles
Magnitude of purification was calculated by dividing the specific activity in the BBM fraction by the specific activity in the initial tissue homogenate
Values are means ± s.e.m. (N=6)