Primer sequences used for first-strand cDNA synthesis, and 3′ and 5′ RACE
| cDNA . | 3′ RACE forward primer . | Product size (bp) . | 5′ RACE reverse primer . | Product size (bp) . |
|---|---|---|---|---|
| MyoD | AGATGCACGTCCACCAACCCGAACC | 1143 | AAGGATCCCCACTTTGGGCAGCCTCTGG | 512 |
| α-actin | ACCTTCCAGCAGATGTGGATCAGCA | 494 | AAGCACTTCCTGTGGACGATGGAGG | 1194 |
| MyHC | CAAGGAGCAGGCTGCTATGGTTGAGC | 934 | ||
| TnT | CCAAGCAGCAGAAGGGCCCGTT | 447 | ||
| TnI | GATGAGGAGCGGTACGATGCTGCGG | 516 | GCAGACATGCGCACCTTCTTCAGGG | 447 |
| CK-M | GGTGGCGATGACCTGGACCCC | 1205 | ||
| TnC | TGCATCAGTACCAAGGA | 606 |
| cDNA . | 3′ RACE forward primer . | Product size (bp) . | 5′ RACE reverse primer . | Product size (bp) . |
|---|---|---|---|---|
| MyoD | AGATGCACGTCCACCAACCCGAACC | 1143 | AAGGATCCCCACTTTGGGCAGCCTCTGG | 512 |
| α-actin | ACCTTCCAGCAGATGTGGATCAGCA | 494 | AAGCACTTCCTGTGGACGATGGAGG | 1194 |
| MyHC | CAAGGAGCAGGCTGCTATGGTTGAGC | 934 | ||
| TnT | CCAAGCAGCAGAAGGGCCCGTT | 447 | ||
| TnI | GATGAGGAGCGGTACGATGCTGCGG | 516 | GCAGACATGCGCACCTTCTTCAGGG | 447 |
| CK-M | GGTGGCGATGACCTGGACCCC | 1205 | ||
| TnC | TGCATCAGTACCAAGGA | 606 |
| . | RT-PCR analysis primers . | . | . | |
|---|---|---|---|---|
| cDNA . | Forward . | Reverse . | Product size (bp) . | |
| MyoD | GAGCCTTGGTCGAACATCACCGAC | GGAACTTTGATGAGCTTGCTTCTTATCAG | 1262 | |
| L15 | GCTCCCAGACCCACCAGACCC | ATGGCCTTGTGGAAGGTGTCG | 311 | |
| . | RT-PCR analysis primers . | . | . | |
|---|---|---|---|---|
| cDNA . | Forward . | Reverse . | Product size (bp) . | |
| MyoD | GAGCCTTGGTCGAACATCACCGAC | GGAACTTTGATGAGCTTGCTTCTTATCAG | 1262 | |
| L15 | GCTCCCAGACCCACCAGACCC | ATGGCCTTGTGGAAGGTGTCG | 311 | |
MyoD, Myogenic determination factor; α-actin,skeletal α-actin; MyHC, myosin heavy chain; TnT,troponin T; TnI, troponin I; CK-M, muscle creatine kinase; TnC, troponin C
RACE, rapid amplification of cDNA ends; RT-PCR, reverse transcriptase-polymerase chain reaction. For details, see Materials and methods
Final product sizes calculated include the primer sequences
Clones used for in situ hybridization