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Table 3.

The effect of synthetic leucokinin-VIII on transepithelial electrolyte and fluid secretion in isolated Malpighian tubules of Aedes aegypti

Experimental condition (N tubules)	Fluid secretion (nl min⁻¹)	[Na⁺]_sf (mmol l⁻¹)	[K⁺]_sf (mmol l⁻¹)	[Cl⁻]_sf (mmol l⁻¹)
Control (10)	0.49±0.04	141.8±9.8	64.9±9.1	204.9±4.7
Leucokinin-VIII (10)	0.91±0.08 ^*	100.5±10.7 ^*	84.8±11.2 ^*	193.5±3.5
		Na⁺ secretion (pmol min⁻¹)	K⁺ secretion (pmol min⁻¹)	Cl⁻ secretion (pmol min⁻¹)
Control (44)		69.9±8.4	29.3±3.3	100.1±8.7
Leucokinin-VIII (10)		96.0±16.2 ^*	72.5±9.3 ^*	177.9±16.8 ^*

Experimental condition (N tubules)	Fluid secretion (nl min⁻¹)	[Na⁺]_sf (mmol l⁻¹)	[K⁺]_sf (mmol l⁻¹)	[Cl⁻]_sf (mmol l⁻¹)
Control (10)	0.49±0.04	141.8±9.8	64.9±9.1	204.9±4.7
Leucokinin-VIII (10)	0.91±0.08 ^*	100.5±10.7 ^*	84.8±11.2 ^*	193.5±3.5
		Na⁺ secretion (pmol min⁻¹)	K⁺ secretion (pmol min⁻¹)	Cl⁻ secretion (pmol min⁻¹)
Control (44)		69.9±8.4	29.3±3.3	100.1±8.7
Leucokinin-VIII (10)		96.0±16.2 ^*	72.5±9.3 ^*	177.9±16.8 ^*

The concentrations of Na⁺, K⁺ and Cl⁻ were measured in picoliter volumes of secreted fluid by electron-probe (X-ray) analysis(Pannabecker et al., 1993). Ion secretion rates are the product of fluid secretion and molar concentration. Peritubular concentrations were 151.8 mmol l⁻¹, 3.4 mmol l⁻¹ and 158.0 mmol l⁻¹ for Na⁺, K⁺ and Cl⁻, respectively. sf, secreted fluid. ^*P<0.05.

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