Effect of in organello treatments on decay rates (k) and half-lives (t1/2) *of various mitochondrial genes using data from dot-blot analyses
| . | Control . | Anoxia+low pH . | Anoxia . | Anoxia+1.5 mmol l−1 ATP . | Low pH . |
|---|---|---|---|---|---|
| ATP6 | |||||
| k† | 1.44×10−2 | 6.22×10−4 | 5.90×10−3 | 9.67×10−4 | 5.49×10−3 |
| t1/2† | 48.2 | 1113 | 117 | 716 | 187 |
| COX I | |||||
| k | 6.75×10−3 | 9.58×10−4 | 1.90×10−3 | 3.65×10−3 | 5.49×10−3 |
| t1/2 | 102 | 723 | 364 | 194 | 126 |
| CYB | |||||
| k | 1.03×10−2 | 1.37×10−3 | NA | 2.75×10−3 | 2.62×10−3 |
| t1/2 | 67.5 | 506 | NA | 252 | 264 |
| ND1 | |||||
| k | 1.48×10−2 | NA | 4.16×10−3 | 8.45×10−4 | 1.99×10−3 |
| t½ | 47.0 | NA | 167 | 820 | 346 |
| . | Control . | Anoxia+low pH . | Anoxia . | Anoxia+1.5 mmol l−1 ATP . | Low pH . |
|---|---|---|---|---|---|
| ATP6 | |||||
| k† | 1.44×10−2 | 6.22×10−4 | 5.90×10−3 | 9.67×10−4 | 5.49×10−3 |
| t1/2† | 48.2 | 1113 | 117 | 716 | 187 |
| COX I | |||||
| k | 6.75×10−3 | 9.58×10−4 | 1.90×10−3 | 3.65×10−3 | 5.49×10−3 |
| t1/2 | 102 | 723 | 364 | 194 | 126 |
| CYB | |||||
| k | 1.03×10−2 | 1.37×10−3 | NA | 2.75×10−3 | 2.62×10−3 |
| t1/2 | 67.5 | 506 | NA | 252 | 264 |
| ND1 | |||||
| k | 1.48×10−2 | NA | 4.16×10−3 | 8.45×10−4 | 1.99×10−3 |
| t½ | 47.0 | NA | 167 | 820 | 346 |
ATP6, ATP synthase; COX1, cytochrome oxidase; CYB, cytochrome b;ND1, NADH dehydrogenase.
NA, increases in RNA amounts (although not statistically significant) make these assessments inapplicable.
Decay rates were calculated according to the formula for second-order decay kinetics: k=2.3/(time2-time1)×log(RNA1/RNA2). Half-life was calculated according to the equation t½=0.693/k(Segel, 1976).
k, min−1; t½, min.