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Table 1.

Kinetics of substrate transport for excitatory amino acid transporters(EAATs)

Km (μmol 1-)
Proteinl-Glutamatel-Aspartated-Aspartate
AeaEAAT 29.4±4.6 10.1±0.9 13.3±3.0 
dEAAT1a 72±3 31±4 92±9 
dEAAT2b 184.9±12.4 33.7±6.2 29.2±6.1 
TrnEAAT 39.4±3.1  20.4 
DipEAAT 30.5±4.7  222.4 
hEAAT1c 48±10  60±12 
hEAAT2c 97±4  54±9 
hEAAT3c 62±8  47±9 
Km (μmol 1-)
Proteinl-Glutamatel-Aspartated-Aspartate
AeaEAAT 29.4±4.6 10.1±0.9 13.3±3.0 
dEAAT1a 72±3 31±4 92±9 
dEAAT2b 184.9±12.4 33.7±6.2 29.2±6.1 
TrnEAAT 39.4±3.1  20.4 
DipEAAT 30.5±4.7  222.4 
hEAAT1c 48±10  60±12 
hEAAT2c 97±4  54±9 
hEAAT3c 62±8  47±9 

Aea, Aedes aegypti; d, Drosophila melanogaster; Trn, Trichoplusia ni; Dip, Diploptera punctata; h, human.

HeLa cells infected with vTF7-3 recombinant vaccinia virus and transfected with pBSAMVBC10 were assayed for transport of 30 nmol 1-13H-labeled and varying concentrations (0-500 μmol 1-1) of unlabeled substrate for 5 min.

Affinity (Km) values shown are means ± s.e.m. of 3-4 individual experiments and are compared to published values from orthologues.

Corresponding Vmax values were 6.0, 3.0 and 6.9 fmol cell-1 h-1 for l-Glu, l-Asp and d-Asp, respectively.

a

Seal et al. (1998)expression in COS-7 cells.

b

Besson et al. (2000)expression in S2 cells.

c

Arriza et al. (1994) expression in COS-7 cells.

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