. | Km (μmol 1-) . | . | . | ||
---|---|---|---|---|---|
Protein . | l-Glutamate . | l-Aspartate . | d-Aspartate . | ||
AeaEAAT | 29.4±4.6 | 10.1±0.9 | 13.3±3.0 | ||
dEAAT1a | 72±3 | 31±4 | 92±9 | ||
dEAAT2b | 184.9±12.4 | 33.7±6.2 | 29.2±6.1 | ||
TrnEAAT | 39.4±3.1 | 20.4 | |||
DipEAAT | 30.5±4.7 | 222.4 | |||
hEAAT1c | 48±10 | 60±12 | |||
hEAAT2c | 97±4 | 54±9 | |||
hEAAT3c | 62±8 | 47±9 |
. | Km (μmol 1-) . | . | . | ||
---|---|---|---|---|---|
Protein . | l-Glutamate . | l-Aspartate . | d-Aspartate . | ||
AeaEAAT | 29.4±4.6 | 10.1±0.9 | 13.3±3.0 | ||
dEAAT1a | 72±3 | 31±4 | 92±9 | ||
dEAAT2b | 184.9±12.4 | 33.7±6.2 | 29.2±6.1 | ||
TrnEAAT | 39.4±3.1 | 20.4 | |||
DipEAAT | 30.5±4.7 | 222.4 | |||
hEAAT1c | 48±10 | 60±12 | |||
hEAAT2c | 97±4 | 54±9 | |||
hEAAT3c | 62±8 | 47±9 |
Aea, Aedes aegypti; d, Drosophila melanogaster; Trn, Trichoplusia ni; Dip, Diploptera punctata; h, human.
HeLa cells infected with vTF7-3 recombinant vaccinia virus and transfected with pBSAMVBC10 were assayed for transport of 30 nmol 1-13H-labeled and varying concentrations (0-500 μmol 1-1) of unlabeled substrate for 5 min.
Affinity (Km) values shown are means ± s.e.m. of 3-4 individual experiments and are compared to published values from orthologues.
Corresponding Vmax values were 6.0, 3.0 and 6.9 fmol cell-1 h-1 for l-Glu, l-Asp and d-Asp, respectively.
Seal et al. (1998)expression in COS-7 cells.
Besson et al. (2000)expression in S2 cells.
Arriza et al. (1994) expression in COS-7 cells.