Comparison of branchial ammonia excretion rate calculated from Equation 4 to the measured net excretion rate from fish prior to (-0.5 h) and after 96 h exposure to CLP conditions
| . | Time after addition of test water (h) . | . | |
|---|---|---|---|
| . | -0.5 . | 96 . | |
| Measured ṀNH3 (μmol N kg-1 h-1) | 137.2±16.4 | 342.1±21.8 | |
| Δ Tamm(v̄—a) (μmol l-1) | 69.8±12.8 | 82.8±16.7 | |
| Cardiac output (ml min-1 kg-1) | 34.5 | 45.8 | |
| Calculated ṀNH3 (μmol N kg-1 h-1) | 144.2±24.7 | 227.6±51.8§ | |
| Calculated ṀNH3/ Measured ṀNH3 | 0.95±0.1 | 0.66±0.1 | |
| . | Time after addition of test water (h) . | . | |
|---|---|---|---|
| . | -0.5 . | 96 . | |
| Measured ṀNH3 (μmol N kg-1 h-1) | 137.2±16.4 | 342.1±21.8 | |
| Δ Tamm(v̄—a) (μmol l-1) | 69.8±12.8 | 82.8±16.7 | |
| Cardiac output (ml min-1 kg-1) | 34.5 | 45.8 | |
| Calculated ṀNH3 (μmol N kg-1 h-1) | 144.2±24.7 | 227.6±51.8§ | |
| Calculated ṀNH3/ Measured ṀNH3 | 0.95±0.1 | 0.66±0.1 | |
CLP conditions: pH 5, 0.08 μmol l-1 copper.
Mean value for cardiac output was the Routine value in Table 1.
indicates a significant difference between observed and expected values (χ2 test, d.f.=4, P=0.05, N=6).