Percentage of female, male and abnormal gonads as determined by histological examination in eelpout (Zoarces viviparus) embryos after 35 days of exposure to 4-tert-octylphenol or 17β-oestradiol
| . | Day 0 (11 Oct) . | Day 35 (15 Nov) . | . | . | . | |||
|---|---|---|---|---|---|---|---|---|
| . | Control . | Control . | OP25 . | OP100 . | E2 . | |||
| Number of mother fish | 10 | 8 | 8 | 7 | 8 | |||
| Number of embryos analysed* | 48 | 44 | 43 | 40 | 47 | |||
| Percentage of total number of gonads of the embryos | ||||||||
| Female gonad (early differentiating ovary) | 48 | 0 | 0 | 0 | 0 | |||
| Female gonad (ovary with oocytes) | 0 | 52 | 58 | 46 | 49 | |||
| Male gonad (presumptive) | 52 | 48 | 42 | 22 | 51 | |||
| Abnormal gonad — male or female? | 0 | 0 | 0 | 32 | 0 | |||
| . | Day 0 (11 Oct) . | Day 35 (15 Nov) . | . | . | . | |||
|---|---|---|---|---|---|---|---|---|
| . | Control . | Control . | OP25 . | OP100 . | E2 . | |||
| Number of mother fish | 10 | 8 | 8 | 7 | 8 | |||
| Number of embryos analysed* | 48 | 44 | 43 | 40 | 47 | |||
| Percentage of total number of gonads of the embryos | ||||||||
| Female gonad (early differentiating ovary) | 48 | 0 | 0 | 0 | 0 | |||
| Female gonad (ovary with oocytes) | 0 | 52 | 58 | 46 | 49 | |||
| Male gonad (presumptive) | 52 | 48 | 42 | 22 | 51 | |||
| Abnormal gonad — male or female? | 0 | 0 | 0 | 32 | 0 | |||
Abbreviations: 4-tOP, 4-tert-octylphenol; OP25, 25 μg l-1 4-tOP; OP100, 100 μg l-1 4-tOP; E2,0.5 μg l-1 17β-oestradiol.
4-6 embryos from each mother fish were processed and analysed by light microscopy.