Yeast strains used in this study
| Strain . | Genotype . | Source . |
|---|---|---|
| X2180-1B | MATα gal2 SUC2 mal mel CUP1 (wild type) | YGSC1 |
| RSY 248 | MATα his4-619 (wild type) | R. Schekman2 |
| RSY 249 | MATa, his 4-619, ura3-52 (wild type) | R. Schekman |
| SEY 5186 | MATα sec 18-1 ura3-52 leu2-3, 112 | E. C. Gaynor and S. D. Emr3 |
| ASHY 1896-1 | MATα sec 18-1 ura3-52 leu2-3, 112(pYES2::URA3) | This study |
| ASHY 1896-2 | MATα sec 18-1 ura3-52 leu2-3, 112(PGAL-MsNSF::URA3) | This study |
| ASHY 1896-5 | MATα sec 18-1 ura3-52 leu2-3, 112(SEC18::URA3) | This study |
| ASHY 1896-7 | MATα sec 18-1 ura3-52 leu2-3, 112(PGAL-SEC18::URA3) | This study |
| RSY 271 | MATα sec 18-1 ura3-52 his4-619 | R. Schekman |
| ASHY 1896-9 | MATα sec 18-1 ura3-52 his4-619 (pYES2::URA3) | This study |
| ASHY 1896-10 | MATα sec 18-1 ura3-52 his4-619 (PGAL-MsNSF::URA3) | This study |
| RSY 319 | MATα sec 18-2 leu2-3 112 | R. Schekman |
| RSY 272 | MATa, his 4-619, ura3-52, sec 18-1 | R. Schekman |
| ASHY 18a-11 | MATa, his 4-619, ura3-52, sec 18-1 (pYES2::URA3) | This study |
| ASHY 18a-12 | MATa, his 4-619, ura3-52, sec 18-1 (SEC18::URA3) | This study |
| ASHY 18a-13 | MATa, his 4-619, ura3-52, sec 18-1(PGAL-SEC18::URA3) | This study |
| ASHY 18a-14 | MATa, his 4-619, ura3-52, sec 18-1(PGAL-MsNSF::URA3) | This study |
| SEY 5188 | MATα sec 18-1 ura3-52 leu2-3 112 suc 2Δ9 | E. C. Gaynor and S. D. Emr |
| ASHY 18-1 | MATα sec 18-1 ura3-52 leu2-3,112 suc 2Δ9(pYES2::URA3) | This study |
| ASHY 18-2 | MATα sec 18-1 ura3-52 leu2-3,112 suc 2Δ9(PGAL- MsNSF::URA3) | This study |
| ASHY 18-3 | MATα sec 18-1 ura3-52 leu2-3,112 suc 2Δ9(SEC18::URA3) | This study |
| ASHY 18-4 | MATα sec 18-1 ura3-52 leu2-3,112 suc 2Δ9(PGAL-SEC18::URA3) | This study |
| EGY 1181-5 | MATα sec 18-1 ura3-52 leu2-3,112 his3suc2Δ9 | E. C. Gaynor and S. D. Emr |
| ASHY 1181-1 | MATα sec 18-1 ura3-52 leu2-3,112 his3suc2Δ9 (pYES2::URA3) | This study |
| ASHY 1181-2 | MATα sec 18-1 ura3-52 leu2-3,112 his3suc2Δ9 (PGAL- MsNSF::URA3) | This study |
| ASHY 1181-3 | MATα sec 18-1 ura3-52 leu2-3,112 his3suc2Δ9 (SEC18::URA3) | This study |
| ASHY 1181-4 | MATα sec 18-1 ura3-52 leu2-3,112 his3suc2Δ9 (PGAL-SEC18::URA3) | This study |
| RH 448 | MATa ura3, leu2, his4, lys2, bar1-1 | H. Riezman4 |
| RH 1737 | MATa sec 18-20, ura3, leu2, his4, bar1-1 | H. Riezman |
| Strain . | Genotype . | Source . |
|---|---|---|
| X2180-1B | MATα gal2 SUC2 mal mel CUP1 (wild type) | YGSC1 |
| RSY 248 | MATα his4-619 (wild type) | R. Schekman2 |
| RSY 249 | MATa, his 4-619, ura3-52 (wild type) | R. Schekman |
| SEY 5186 | MATα sec 18-1 ura3-52 leu2-3, 112 | E. C. Gaynor and S. D. Emr3 |
| ASHY 1896-1 | MATα sec 18-1 ura3-52 leu2-3, 112(pYES2::URA3) | This study |
| ASHY 1896-2 | MATα sec 18-1 ura3-52 leu2-3, 112(PGAL-MsNSF::URA3) | This study |
| ASHY 1896-5 | MATα sec 18-1 ura3-52 leu2-3, 112(SEC18::URA3) | This study |
| ASHY 1896-7 | MATα sec 18-1 ura3-52 leu2-3, 112(PGAL-SEC18::URA3) | This study |
| RSY 271 | MATα sec 18-1 ura3-52 his4-619 | R. Schekman |
| ASHY 1896-9 | MATα sec 18-1 ura3-52 his4-619 (pYES2::URA3) | This study |
| ASHY 1896-10 | MATα sec 18-1 ura3-52 his4-619 (PGAL-MsNSF::URA3) | This study |
| RSY 319 | MATα sec 18-2 leu2-3 112 | R. Schekman |
| RSY 272 | MATa, his 4-619, ura3-52, sec 18-1 | R. Schekman |
| ASHY 18a-11 | MATa, his 4-619, ura3-52, sec 18-1 (pYES2::URA3) | This study |
| ASHY 18a-12 | MATa, his 4-619, ura3-52, sec 18-1 (SEC18::URA3) | This study |
| ASHY 18a-13 | MATa, his 4-619, ura3-52, sec 18-1(PGAL-SEC18::URA3) | This study |
| ASHY 18a-14 | MATa, his 4-619, ura3-52, sec 18-1(PGAL-MsNSF::URA3) | This study |
| SEY 5188 | MATα sec 18-1 ura3-52 leu2-3 112 suc 2Δ9 | E. C. Gaynor and S. D. Emr |
| ASHY 18-1 | MATα sec 18-1 ura3-52 leu2-3,112 suc 2Δ9(pYES2::URA3) | This study |
| ASHY 18-2 | MATα sec 18-1 ura3-52 leu2-3,112 suc 2Δ9(PGAL- MsNSF::URA3) | This study |
| ASHY 18-3 | MATα sec 18-1 ura3-52 leu2-3,112 suc 2Δ9(SEC18::URA3) | This study |
| ASHY 18-4 | MATα sec 18-1 ura3-52 leu2-3,112 suc 2Δ9(PGAL-SEC18::URA3) | This study |
| EGY 1181-5 | MATα sec 18-1 ura3-52 leu2-3,112 his3suc2Δ9 | E. C. Gaynor and S. D. Emr |
| ASHY 1181-1 | MATα sec 18-1 ura3-52 leu2-3,112 his3suc2Δ9 (pYES2::URA3) | This study |
| ASHY 1181-2 | MATα sec 18-1 ura3-52 leu2-3,112 his3suc2Δ9 (PGAL- MsNSF::URA3) | This study |
| ASHY 1181-3 | MATα sec 18-1 ura3-52 leu2-3,112 his3suc2Δ9 (SEC18::URA3) | This study |
| ASHY 1181-4 | MATα sec 18-1 ura3-52 leu2-3,112 his3suc2Δ9 (PGAL-SEC18::URA3) | This study |
| RH 448 | MATa ura3, leu2, his4, lys2, bar1-1 | H. Riezman4 |
| RH 1737 | MATa sec 18-20, ura3, leu2, his4, bar1-1 | H. Riezman |