The Company of Biologists

Table 1.

Experimental time-course

	Day
	1	2	3	4	5	6	7	8	9	10	11
Put in vials	5ht	oa	wtb	mb	da	b/e
	wtb	da	b/e	5ht	oa	mb
Mark					5ht	oa	wtb	mb	da	b/e
					wtb	da	b/e	5ht	oa	mb
Record						5ht	oa	wtb	mb	da	b/e
						wtb	da	b/e	5ht	oa	mb

	Day
	1	2	3	4	5	6	7	8	9	10	11
Put in vials	5ht	oa	wtb	mb	da	b/e
	wtb	da	b/e	5ht	oa	mb
Mark					5ht	oa	wtb	mb	da	b/e
					wtb	da	b/e	5ht	oa	mb
Record						5ht	oa	wtb	mb	da	b/e
						wtb	da	b/e	5ht	oa	mb

Two groups were treated in separate vials but in parallel each experimental day. Each group was treated in two replicates, starting with different flies on different days.

For abbreviations see Materials and methods.

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