Antioxidant enzyme activities and lipid-peroxidation levels in liver and heart of the sturgeon Acipenser naccarii during acclimation to seawater (35‰) and after a period of 20 days at this salinity
| . | Salinity (‰) . | . | . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|
| . | 15 . | 22 . | 29 . | 35 . | 35* . | ||||
| Liver | |||||||||
| CAT (U mg protein-1) | 487.8±44.7a | 324.2±12.5b | 302.8±13.9b,c | 318.8±17.7b | 235.6±21.6c | ||||
| GPX (U mg protein-1) | 30.0±4.0 | 30.0±4.0 | 20.0±3.0 | 30.0±4.0 | 22.0±2.0 | ||||
| SOD (U mg protein-1) | 12.7±0.8a | 9.8±0.4b | 9.3±0.9b | 10.2±0.9b | 7.0±0.1c | ||||
| MDA (nmol ml-1) | 2.1±0.2 | 1.2±0.2 | 1.3±0.2 | 1.4±0.1 | 1.4±0.2 | ||||
| Heart | |||||||||
| CAT (U mg protein-1) | 11.6±0.5 | 11.2±0.6 | 9.2±0.2 | 9.1±0.9 | 12.8±1.6 | ||||
| GPX (U mg protein-1) | 7.8±0.8 | 7.2±0.8 | 8.0±0.1 | 6.0±0.6 | 7.0±0.3 | ||||
| SOD (U mg protein-1) | 11.1±0.5 | 9.6±0.8 | 9.5±0.8 | 8.5±0.5 | 10.2±0.7 | ||||
| MDA (nmol ml-1) | 1.0±0.2 | 0.9±0.2 | 0.6±0.2 | 0.4±0.1 | 1.2±0.2 | ||||
| . | Salinity (‰) . | . | . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|
| . | 15 . | 22 . | 29 . | 35 . | 35* . | ||||
| Liver | |||||||||
| CAT (U mg protein-1) | 487.8±44.7a | 324.2±12.5b | 302.8±13.9b,c | 318.8±17.7b | 235.6±21.6c | ||||
| GPX (U mg protein-1) | 30.0±4.0 | 30.0±4.0 | 20.0±3.0 | 30.0±4.0 | 22.0±2.0 | ||||
| SOD (U mg protein-1) | 12.7±0.8a | 9.8±0.4b | 9.3±0.9b | 10.2±0.9b | 7.0±0.1c | ||||
| MDA (nmol ml-1) | 2.1±0.2 | 1.2±0.2 | 1.3±0.2 | 1.4±0.1 | 1.4±0.2 | ||||
| Heart | |||||||||
| CAT (U mg protein-1) | 11.6±0.5 | 11.2±0.6 | 9.2±0.2 | 9.1±0.9 | 12.8±1.6 | ||||
| GPX (U mg protein-1) | 7.8±0.8 | 7.2±0.8 | 8.0±0.1 | 6.0±0.6 | 7.0±0.3 | ||||
| SOD (U mg protein-1) | 11.1±0.5 | 9.6±0.8 | 9.5±0.8 | 8.5±0.5 | 10.2±0.7 | ||||
| MDA (nmol ml-1) | 1.0±0.2 | 0.9±0.2 | 0.6±0.2 | 0.4±0.1 | 1.2±0.2 | ||||
Values are means ± S.E.M. Values with different superscript letters within each row are significantly different (analysis of variance, P<0.05, N=5 fish at each salinity).
Catalase (CAT) activity, 1 unit (U) = 1 mmol min-1; glutathione peroxidase (GPX) activity, 1 unit = 1 nmol min-1; superoxide dismutase (SOD) activity, 1 unit = 50% inhibition of cytochrome creduction min-1; lipid peroxidation levels are expressed as malondialdehyde (MDA) concentration (nmol MDA ml-1).
After a period of 20 days at 35‰.