Amplitude of intracellular Ca2+ concentration([Ca2+]i) (nmol l-1) in trout atrial myocytes
. | SQ0.2Hz . | SQphysiol . | APphysiol . |
---|---|---|---|
7°C | 474±53a,e | 662±65a,f | 234±49a,g |
14°C | 398±40a,e | 506±48a,f | 122±41b,g |
21°C | 198±27b,e | 278±41b,f | 79±12b,g |
. | SQ0.2Hz . | SQphysiol . | APphysiol . |
---|---|---|---|
7°C | 474±53a,e | 662±65a,f | 234±49a,g |
14°C | 398±40a,e | 506±48a,f | 122±41b,g |
21°C | 198±27b,e | 278±41b,f | 79±12b,g |
The effect of temperature and the frequency/stimulus waveform on the amplitude of the stimulus-induced change in [Ca2+]i,calculated as the difference between the diastolic and systolic Ca2+ concentration with each depolarization. Values are means of 4-8 cells at each temperature.
The frequency of stimulation during SQphysiol (square pulses)and APphysiol (action potential) was 0.6 Hz at 7°C, 1.0 Hz at 14°C and 1.4 Hz at 21°C.
The effect of temperature is indicated by dissimilar letters a, b and c(compare vertically within each frequency/stimulus treatment) [analysis of variance (ANOVA), Student—Newman—Keuls (SNK) test]. Dissimilar letters e, f and g indicate significant differences between frequency/stimulus protocols (compare horizontally within each temperature) [repeated measures ANOVA, SNK].