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Table 3.

Effects of 17β-estradiol (E₂) on mature female squirrelfish

	Day 5			Day 6
	Control	E₂ treated	Control	E₂ treated	Control	E₂ treated
Liver zinc	2.32±2.84	3.36±0.69	6.12±1.06	3.61±1.01	7.13±1.83	14.2±6.35
VTG	0.43±0.27	33.0±2.82^*	0.55±0.27	24.9±8.66^*	0.13±0.06	12.1±2.12^*
E₂	1.17±0.08	11.4±5.10^*	2.53±0.95	18.2±8.95^*	1.61±0.21	0.13±0.04^*
T	2.92±0.28	0.10±0.03^*	3.06±0.13	0.09±0.05^*	2.88±0.29	0.25±0.14^*
P	31.2±8.51	3.95±0.52^*	32.0±3.31	3.67±0.83^*	36.1±3.65	3.47±0.77^*
MT mRNA	1.03±0.18	0.82±0.10	1.01±0.13	0.72±0.11	0.98±0.24	0.93±0.14
C MT	1.29±0.60	0.48±0.05^*	0.63±0.20	1.01±0.08	0.64±0.09	0.81±0.04

	Day 5			Day 6
	Control	E₂ treated	Control	E₂ treated	Control	E₂ treated
Liver zinc	2.32±2.84	3.36±0.69	6.12±1.06	3.61±1.01	7.13±1.83	14.2±6.35
VTG	0.43±0.27	33.0±2.82^*	0.55±0.27	24.9±8.66^*	0.13±0.06	12.1±2.12^*
E₂	1.17±0.08	11.4±5.10^*	2.53±0.95	18.2±8.95^*	1.61±0.21	0.13±0.04^*
T	2.92±0.28	0.10±0.03^*	3.06±0.13	0.09±0.05^*	2.88±0.29	0.25±0.14^*
P	31.2±8.51	3.95±0.52^*	32.0±3.31	3.67±0.83^*	36.1±3.65	3.47±0.77^*
MT mRNA	1.03±0.18	0.82±0.10	1.01±0.13	0.72±0.11	0.98±0.24	0.93±0.14
C MT	1.29±0.60	0.48±0.05^*	0.63±0.20	1.01±0.08	0.64±0.09	0.81±0.04

Effects of 17β-estradiol (E₂) on mature female squirrelfish in terms of liver zinc, plasma vitellogenin (VTG), testosterone (T),17β-estradiol (E₂) and progesterone (P) levels; hepatic metallothionein mRNA levels (MT mRNA); and MT protein levels in cytosolic (C)liver fractions.Other details as in Table 2.

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