Skip to Main Content
Table 2.

Blood oxygen affinity in vitro at twoPCO2levels (2% and 5% CO2) in fasting and digesting snakes (Python molurus)

ExperimentBefore feeding (0h)After feeding (48h)Control (0h)Control (48h)
CO2 level2% CO25% CO22% CO25% CO22% CO25% CO22% CO25% CO2
pH 7.57±0.02 (9) 7.31±0.02(9) 7.65±0.03(9)* 7.40±0.02(7),* 7.63±0.02 (5) 7.34±0.02(5) 7.66±0.03(4)* 7.37±0.02(4) 
P50 (kPa) 4.67±0.07 (9) 5.36±0.10(9) 3.42±0.19(9)* 4.43±0.31(7),* 4.39±0.12 (5) 5.38±0.19(5) 4.40±0.19 (4) 5.38±0.23(4) 
logP50 0.67±0.01 (9) 0.73±0.01(9) 0.53±0.02(9)* 0.64±0.04(7),* 0.64±0.01 (5) 0.73±0.01(5) 0.64±0.02 (4) 0.73±0.02(4) 
n 2.86±0.16 (9) 2.64±0.14(9) 2.42±0.17(9)* 2.36±0.15 (7) 2.88±0.10 (5) 2.47±0.13(5) 2.47±0.20 (4) 2.50±0.02 (4) 
Bohr effect (Φ) -0.23±0.02 (9)  -0.36±0.03(7)*  -0.31±0.02 (5)  -0.30±0.01 (4)  
ExperimentBefore feeding (0h)After feeding (48h)Control (0h)Control (48h)
CO2 level2% CO25% CO22% CO25% CO22% CO25% CO22% CO25% CO2
pH 7.57±0.02 (9) 7.31±0.02(9) 7.65±0.03(9)* 7.40±0.02(7),* 7.63±0.02 (5) 7.34±0.02(5) 7.66±0.03(4)* 7.37±0.02(4) 
P50 (kPa) 4.67±0.07 (9) 5.36±0.10(9) 3.42±0.19(9)* 4.43±0.31(7),* 4.39±0.12 (5) 5.38±0.19(5) 4.40±0.19 (4) 5.38±0.23(4) 
logP50 0.67±0.01 (9) 0.73±0.01(9) 0.53±0.02(9)* 0.64±0.04(7),* 0.64±0.01 (5) 0.73±0.01(5) 0.64±0.02 (4) 0.73±0.02(4) 
n 2.86±0.16 (9) 2.64±0.14(9) 2.42±0.17(9)* 2.36±0.15 (7) 2.88±0.10 (5) 2.47±0.13(5) 2.47±0.20 (4) 2.50±0.02 (4) 
Bohr effect (Φ) -0.23±0.02 (9)  -0.36±0.03(7)*  -0.31±0.02 (5)  -0.30±0.01 (4)  

Data for snakes that were not fed but sampled at a 48 h interval are also included (unfed control).

pH, pH measured in the tonometers; P50, oxygen tension at half saturation; n, Hills coefficient for cooperativity of oxygen binding; Bohr effect (Φ), calculated as▵logP50/▵pH. Values are means ± S.E.M.(N).

*

signifies statistical difference between values at 0 h and 48 h; signifies statistical difference between values at 2% and 5% CO2 within each experimental condition.

Close Modal

or Create an Account

Close Modal
Close Modal