The Company of Biologists

Table 2.

cDNA clusters from a salivary gland library from adult femaleIxodes scapularis ticks probably associated with secretory products

Line number	Cluster number	Number of sequences	Best BlastX match to NR database^¹	E-value^²	Best RPSblast to CDD database^³	E-value^²	Comments	New?^⁴
1	1	44	gi\|15428308\| 14 kDa salivary...	7.00E-34	pfam02414 Borrelia_orfA	1.00E-09	14 kDa salivary protein
2	329	1	gi\|15428294\| 15 kDa salivary...	4.00E-35			15 kDa salivary protein
3	364	1	gi\|15428296\| 16 kDa salivary...	1.00E-31			16 kDa salivary protein
4	9	9	gi\|15428310\| 25 kDa salivary...	1.00E-105			25 kDa antigen
5	283	1	gi\|15428304\| 26 kDa salivary...	9.00E-41			26 kDa protein A (new member?)
6	291	1	gi\|7521905\|gi\|7521905\|pir‖T18544	4.00E-41	pfam00207 A2M	3.00E-25	Alpha-2-macroglobulin	Y
7	27	3	gi\|15428300\| 20 kDa salivary...	3.00E-62			Anticomplement protein
8	379	1	gi\|103012\|gi\|103012\|pir‖A41612	2.00E-22	pfam00450 serine_carbpept	9.00E-26	Carboxypeptidase	Y
9	333	1	gi\|13470791\|gi\|13470791\|ref\|NP_102360.1\|	9.00E-12	LOAD_taz taz	0.002	Collagen-like	Y
10	247	1	gi\|12832479\| putative [Mus musculus]	1.00E-18			Conserved protein	Y
11	377	1	gi\|87763\|gi\|87763\|pir‖JS0027 probable	2.00E-10			Conserved protein	Y
12	62	2			pfam00031 cystatin	8.00E-12	Cystatin	Y
13	109	1	gi\|13623795\| defensin B [Ornithodoros	1.00E-05	Smart smart00505 Knot1	6.00E-07	Defensin — antibacterial peptide	Y
14	65	2	gi\|5911708\| hypothetical protein	1.00E-111	pfam01421 Reprolysin	0.008	Disintegrin protease	Y
15	134	1	gi\|5911708\| hypothetical protein	2.00E-35			Disintegrin protease	Y
16	383	1	gi\|5911708\| hypothetical protein	2.00E-93	pfam01421 Reprolysin	2.00E-04	Disintegrin protease	Y
17	163	1	gi\|5911708\| hypothetical protein	7.00E-11			Disintegrin protease?	Y
18	292	1	gi\|10720060\| (G-INX...	4.00E-16	pfam00876 Innexin	2.00E-16	Gap junction protein	Y
19	306	1			pfam02168 Occludin	1.00E-06	Gap junction protein	Y
20	43	2			pfam02168 Occludin	0.005	Gap junction protein?	Y
21	18	6			pfam02008 His_binding	7.00E-04	Histamine binding domain	Y
22	202	1	gi\|15428292\| histamine bindin...	2.00E-61			Histamine binding protein
23	363	1	gi\|8470378\|gi\|8470378\|sp\|O77421\|HBP2_RHI	1.00E-04	pfam02098 His_binding	0.003	Histamine binding protein	Y
24	89	1	gi\|15077002\| ixolaris [Ixodes...	1.00E-40	Smart smart00131 KU	9.00E-13	Kunitz — Ixolaris
25	133	1	gi\|15077002\| ixolaris [Ixodes...	2.00E-74	pfam00014 Kunitz_BPTI	4.00E-11	Kunitz — Ixolaris
26	168	1	gi\|15077002\| ixolaris [Ixodes...	3.00E-45	Smart smart00131 KU	3.00E-07	Kunitz — Ixolaris
27	4	20	gi\|15428348\| Salp 10 [Ixodes s...	5.00E-34	pfam00014 Kunitz_BPTI	0.001	Kunitz — Salp10
28	42	2	gi\|15428348\| Salp10 [Ixodes s...	2.00E-18	pfam00014 Kunitz_BPTI	6.00E-05	Kunitz — Salp10
29	7	14	gi\|124810\|...	0.01	Smart smart00131 KU	1.00E-06	Kunitz domain	Y
30	16	7			Smart smart00131 KU	4.00E-05	Kunitz domain	Y
31	17	6	gi\|3881447\| contains similarity to Pfam	0.018	Smart smart00131 KU	1.00E-04	Kunitz domain	Y
32	24	3			Smart smart00131 KU	0.007	Kunitz domain	Y
33	26	3	gi\|7506657\|gi\|7506657\|pir‖T32060	1.00E-05	Smart smart00131 KU	2.00E-09	Kunitz domain	Y
34	31	3	gi\|7497910\|gi\|7497910\|pir‖T20219	7.00E-06	Smart smart00131 KU	4.00E-08	Kunitz domain	Y
35	45	2			pfam0014 Kunitz_BPTI	6.00E-06	Kunitz domain	Y
36	48	2	gi\|7505725\|gi\|7505725\|pir‖T23573	4.00E-05	pfam00014 Kunitz_BPTI	2.00E-08	Kunitz domain	Y
37	51	2	gi\|10726827\| CG18436 gene product	0.003	Smart smart00131 KU	7.00E-08	Kunitz domain	Y
38	54	2	gi\|7500203\|gi\|7500203\|pir‖T16210	0.066	pfam00014 Kunitz_BPTI	9.00E-05	Kunitz domain	Y
39	68	2	gi\|10726827\| CG18436 gene product	3.00E-07	Smart smart00131 KU	3.00E-04	Kunitz domain	Y
40	73	1	gi\|7500203\|gi\|7500203\|pir‖T16210	9.00E-05	pfam00014 Kunitz_BPTI	2.00E-10	Kunitz domain	Y
41	92	1			Smart smart00131 KU	2.00E-08	Kunitz domain	Y
42	108	1	gi\|7498832\|gi\|7498832\|pir‖T34212	0.014	Smart smart00131 KU	7.00E-06	Kunitz domain	Y
43	124	1			Smart smart00131 KU	4.00E-05	Kunitz domain	Y
44	157	1	gi\|4502167\| precursor protein...	2.00E-07	Smart smart00131 KU	4.00E-12	Kunitz domain	Y
45	176	1			Smart smart00131 KU	0.005	Kunitz domain	Y
46	264	1	gi\|10726827\| CG18436 gene product	6.00E-06	Smart smart00131 KU	6.00E-06	Kunitz domain	Y
47	290	1	gi\|6164595\| lacunin [Manduca...	5.00E-05	Smart smart00131 KU	0.005	Kunitz domain	Y
48	318	1	gi\|400070\|gi\|400070\|sp\|P31713\|ISH1_STOHE	1.00E-14	Smart smart00131 KU	5.00E-22	Kunitz domain	Y
49	323	1	gi\|6164595\| lacunin [Manduca...	4.00E-12	Smart smart00131 KU	7.00E-05	Kunitz domain	Y
50	344	1	gi\|7324126\| Hypothetical protein	0.003	Smart smart00131 KU	7.00E-05	Kunitz domain	Y
51	30	3			pfam00014 Kunitz_BPTI	0.009	Kunitz domain?	Y
52	272	1	gi\|6651241\| TAGL-beta [Mus mu...	3.00E-13	Smart smart00644 Ami_2	2.00E-10	Peptidoglycan recognition protein	Y
53	248	1	gi\|3282590\| peritrophin 1 [Anopheles	3.00E-08	pfam01607 Chitin_bind_2	2.00E-13	Peritrophin	Y
54	145	1	gi\|12002008\| salivary gland 1...	3.00E-21			Salivary 16 kD protein
55	175	1	gi\|12002008\| salivary gland 1...	5.00E-11	pfam02853 ACR	1.00E-04	Salivary 16 kD protein — new member	Y
56	178	1	gi\|12002008\| salivary gland 1...	3.00E-06	pfam00335 transmembrane4	0.003	Salivary 16 kD protein — new member	Y
57	231	1	gi\|15428348\| Salp 10 [Ixodes s...	8.00E-20			Salp 10
58	20	5	gi\|15428348\| Salp10 [Ixodes s...	9.00E-14			Salp10 — new member
59	84	1	gi\|15428348\| Salp10 [Ixodes s...	6.00E-11			Salp10 — new member	Y
60	274	1	gi\|12018322\|gi\|12018322\|ref\|NP_072152.1\|	2.00E-09	Smart smart00020 Tryp_SPc	7.00E-19	Serine protease	Y
61	194	1	gi\|14140097\| hypothetical protein	4.00E-51	Smart smart00093 SERPIN	9.00E-19	Serpin	Y
62	360	1	gi\|862467\| limulus intracellular	3.00E-11	pfam00079 serpin	2.00E-18	Serpin	Y
63	2	41			pfam02326 YMF19	5.00E-04	Short protein	Y
64	5	18			pfam01028 Topoisomerase_I	3.00E-04	Short protein	Y
65	14	7					Short protein	Y
66	15	7					Short protein	Y
67	44	2					Short protein	Y
68	36	2					Short protein?	Y
69	53	2	gi\|11496688\|gi\|11496688\|ref\|NP_045470.1\|	0.001	pfam02118 Srg	2.00E-04	Similarity to Borrelia protein	Y
70	29	3			pfam01391 Collagen	7.00E-04	Small collagen
71	33	3	gi\|15428290\| thrombospondin [...	3.00E-28	pfam02853 ACR	4.00E-05	Thrombospondin
72	233	1	gi\|15428290\| thrombospondin [...	8.00E-30	pfam02853 ACR	9.00E-04	Thrombospondin
73	235	1	gi\|15428290\| thrombospondin [...	1.00E-37			Thrombospondin
74	324	1	gi\|15428290\| thrombospondin [...	5.00E-26	pfam02853 ACR	2.00E-04	Thrombospondin
75	392	1	gi\|15428290\| thrombospondin [...	1.00E-10			Thrombospondin (new member?)
76	279	1			pfam01826 TIL	6.00E-06	Trypsin inhibitor cys rich domain	Y
77	188	1	gi\|14780055\|...	4.00E-07	Smart smart00020 Tryp_SPc	2.00E-08	Trypsin-like protease	Y
78	22	4	gi\|8072217\| Dscam [Drosophila...	0.056				Y
79	23	4						Y
80	39	2						Y
81	58	2						Y
82	61	2						Y
83	67	2						Y
84	95	1						Y
85	102	1						Y
86	105	1						Y
87	122	1						Y
88	123	1						Y
89	130	1						Y
90	137	1						Y
91	212	1						Y
92	229	1						Y
93	258	1						Y
94	261	1						Y
95	277	1						Y
96	296	1						Y
97	353	1						Y
98	359	1						Y
99	375	1						Y
100	389	1			pfam01028 Topoisomerase_I	8.00E-07		Y

Line number	Cluster number	Number of sequences	Best BlastX match to NR database^¹	E-value^²	Best RPSblast to CDD database^³	E-value^²	Comments	New?^⁴
1	1	44	gi\|15428308\| 14 kDa salivary...	7.00E-34	pfam02414 Borrelia_orfA	1.00E-09	14 kDa salivary protein
2	329	1	gi\|15428294\| 15 kDa salivary...	4.00E-35			15 kDa salivary protein
3	364	1	gi\|15428296\| 16 kDa salivary...	1.00E-31			16 kDa salivary protein
4	9	9	gi\|15428310\| 25 kDa salivary...	1.00E-105			25 kDa antigen
5	283	1	gi\|15428304\| 26 kDa salivary...	9.00E-41			26 kDa protein A (new member?)
6	291	1	gi\|7521905\|gi\|7521905\|pir‖T18544	4.00E-41	pfam00207 A2M	3.00E-25	Alpha-2-macroglobulin	Y
7	27	3	gi\|15428300\| 20 kDa salivary...	3.00E-62			Anticomplement protein
8	379	1	gi\|103012\|gi\|103012\|pir‖A41612	2.00E-22	pfam00450 serine_carbpept	9.00E-26	Carboxypeptidase	Y
9	333	1	gi\|13470791\|gi\|13470791\|ref\|NP_102360.1\|	9.00E-12	LOAD_taz taz	0.002	Collagen-like	Y
10	247	1	gi\|12832479\| putative [Mus musculus]	1.00E-18			Conserved protein	Y
11	377	1	gi\|87763\|gi\|87763\|pir‖JS0027 probable	2.00E-10			Conserved protein	Y
12	62	2			pfam00031 cystatin	8.00E-12	Cystatin	Y
13	109	1	gi\|13623795\| defensin B [Ornithodoros	1.00E-05	Smart smart00505 Knot1	6.00E-07	Defensin — antibacterial peptide	Y
14	65	2	gi\|5911708\| hypothetical protein	1.00E-111	pfam01421 Reprolysin	0.008	Disintegrin protease	Y
15	134	1	gi\|5911708\| hypothetical protein	2.00E-35			Disintegrin protease	Y
16	383	1	gi\|5911708\| hypothetical protein	2.00E-93	pfam01421 Reprolysin	2.00E-04	Disintegrin protease	Y
17	163	1	gi\|5911708\| hypothetical protein	7.00E-11			Disintegrin protease?	Y
18	292	1	gi\|10720060\| (G-INX...	4.00E-16	pfam00876 Innexin	2.00E-16	Gap junction protein	Y
19	306	1			pfam02168 Occludin	1.00E-06	Gap junction protein	Y
20	43	2			pfam02168 Occludin	0.005	Gap junction protein?	Y
21	18	6			pfam02008 His_binding	7.00E-04	Histamine binding domain	Y
22	202	1	gi\|15428292\| histamine bindin...	2.00E-61			Histamine binding protein
23	363	1	gi\|8470378\|gi\|8470378\|sp\|O77421\|HBP2_RHI	1.00E-04	pfam02098 His_binding	0.003	Histamine binding protein	Y
24	89	1	gi\|15077002\| ixolaris [Ixodes...	1.00E-40	Smart smart00131 KU	9.00E-13	Kunitz — Ixolaris
25	133	1	gi\|15077002\| ixolaris [Ixodes...	2.00E-74	pfam00014 Kunitz_BPTI	4.00E-11	Kunitz — Ixolaris
26	168	1	gi\|15077002\| ixolaris [Ixodes...	3.00E-45	Smart smart00131 KU	3.00E-07	Kunitz — Ixolaris
27	4	20	gi\|15428348\| Salp 10 [Ixodes s...	5.00E-34	pfam00014 Kunitz_BPTI	0.001	Kunitz — Salp10
28	42	2	gi\|15428348\| Salp10 [Ixodes s...	2.00E-18	pfam00014 Kunitz_BPTI	6.00E-05	Kunitz — Salp10
29	7	14	gi\|124810\|...	0.01	Smart smart00131 KU	1.00E-06	Kunitz domain	Y
30	16	7			Smart smart00131 KU	4.00E-05	Kunitz domain	Y
31	17	6	gi\|3881447\| contains similarity to Pfam	0.018	Smart smart00131 KU	1.00E-04	Kunitz domain	Y
32	24	3			Smart smart00131 KU	0.007	Kunitz domain	Y
33	26	3	gi\|7506657\|gi\|7506657\|pir‖T32060	1.00E-05	Smart smart00131 KU	2.00E-09	Kunitz domain	Y
34	31	3	gi\|7497910\|gi\|7497910\|pir‖T20219	7.00E-06	Smart smart00131 KU	4.00E-08	Kunitz domain	Y
35	45	2			pfam0014 Kunitz_BPTI	6.00E-06	Kunitz domain	Y
36	48	2	gi\|7505725\|gi\|7505725\|pir‖T23573	4.00E-05	pfam00014 Kunitz_BPTI	2.00E-08	Kunitz domain	Y
37	51	2	gi\|10726827\| CG18436 gene product	0.003	Smart smart00131 KU	7.00E-08	Kunitz domain	Y
38	54	2	gi\|7500203\|gi\|7500203\|pir‖T16210	0.066	pfam00014 Kunitz_BPTI	9.00E-05	Kunitz domain	Y
39	68	2	gi\|10726827\| CG18436 gene product	3.00E-07	Smart smart00131 KU	3.00E-04	Kunitz domain	Y
40	73	1	gi\|7500203\|gi\|7500203\|pir‖T16210	9.00E-05	pfam00014 Kunitz_BPTI	2.00E-10	Kunitz domain	Y
41	92	1			Smart smart00131 KU	2.00E-08	Kunitz domain	Y
42	108	1	gi\|7498832\|gi\|7498832\|pir‖T34212	0.014	Smart smart00131 KU	7.00E-06	Kunitz domain	Y
43	124	1			Smart smart00131 KU	4.00E-05	Kunitz domain	Y
44	157	1	gi\|4502167\| precursor protein...	2.00E-07	Smart smart00131 KU	4.00E-12	Kunitz domain	Y
45	176	1			Smart smart00131 KU	0.005	Kunitz domain	Y
46	264	1	gi\|10726827\| CG18436 gene product	6.00E-06	Smart smart00131 KU	6.00E-06	Kunitz domain	Y
47	290	1	gi\|6164595\| lacunin [Manduca...	5.00E-05	Smart smart00131 KU	0.005	Kunitz domain	Y
48	318	1	gi\|400070\|gi\|400070\|sp\|P31713\|ISH1_STOHE	1.00E-14	Smart smart00131 KU	5.00E-22	Kunitz domain	Y
49	323	1	gi\|6164595\| lacunin [Manduca...	4.00E-12	Smart smart00131 KU	7.00E-05	Kunitz domain	Y
50	344	1	gi\|7324126\| Hypothetical protein	0.003	Smart smart00131 KU	7.00E-05	Kunitz domain	Y
51	30	3			pfam00014 Kunitz_BPTI	0.009	Kunitz domain?	Y
52	272	1	gi\|6651241\| TAGL-beta [Mus mu...	3.00E-13	Smart smart00644 Ami_2	2.00E-10	Peptidoglycan recognition protein	Y
53	248	1	gi\|3282590\| peritrophin 1 [Anopheles	3.00E-08	pfam01607 Chitin_bind_2	2.00E-13	Peritrophin	Y
54	145	1	gi\|12002008\| salivary gland 1...	3.00E-21			Salivary 16 kD protein
55	175	1	gi\|12002008\| salivary gland 1...	5.00E-11	pfam02853 ACR	1.00E-04	Salivary 16 kD protein — new member	Y
56	178	1	gi\|12002008\| salivary gland 1...	3.00E-06	pfam00335 transmembrane4	0.003	Salivary 16 kD protein — new member	Y
57	231	1	gi\|15428348\| Salp 10 [Ixodes s...	8.00E-20			Salp 10
58	20	5	gi\|15428348\| Salp10 [Ixodes s...	9.00E-14			Salp10 — new member
59	84	1	gi\|15428348\| Salp10 [Ixodes s...	6.00E-11			Salp10 — new member	Y
60	274	1	gi\|12018322\|gi\|12018322\|ref\|NP_072152.1\|	2.00E-09	Smart smart00020 Tryp_SPc	7.00E-19	Serine protease	Y
61	194	1	gi\|14140097\| hypothetical protein	4.00E-51	Smart smart00093 SERPIN	9.00E-19	Serpin	Y
62	360	1	gi\|862467\| limulus intracellular	3.00E-11	pfam00079 serpin	2.00E-18	Serpin	Y
63	2	41			pfam02326 YMF19	5.00E-04	Short protein	Y
64	5	18			pfam01028 Topoisomerase_I	3.00E-04	Short protein	Y
65	14	7					Short protein	Y
66	15	7					Short protein	Y
67	44	2					Short protein	Y
68	36	2					Short protein?	Y
69	53	2	gi\|11496688\|gi\|11496688\|ref\|NP_045470.1\|	0.001	pfam02118 Srg	2.00E-04	Similarity to Borrelia protein	Y
70	29	3			pfam01391 Collagen	7.00E-04	Small collagen
71	33	3	gi\|15428290\| thrombospondin [...	3.00E-28	pfam02853 ACR	4.00E-05	Thrombospondin
72	233	1	gi\|15428290\| thrombospondin [...	8.00E-30	pfam02853 ACR	9.00E-04	Thrombospondin
73	235	1	gi\|15428290\| thrombospondin [...	1.00E-37			Thrombospondin
74	324	1	gi\|15428290\| thrombospondin [...	5.00E-26	pfam02853 ACR	2.00E-04	Thrombospondin
75	392	1	gi\|15428290\| thrombospondin [...	1.00E-10			Thrombospondin (new member?)
76	279	1			pfam01826 TIL	6.00E-06	Trypsin inhibitor cys rich domain	Y
77	188	1	gi\|14780055\|...	4.00E-07	Smart smart00020 Tryp_SPc	2.00E-08	Trypsin-like protease	Y
78	22	4	gi\|8072217\| Dscam [Drosophila...	0.056				Y
79	23	4						Y
80	39	2						Y
81	58	2						Y
82	61	2						Y
83	67	2						Y
84	95	1						Y
85	102	1						Y
86	105	1						Y
87	122	1						Y
88	123	1						Y
89	130	1						Y
90	137	1						Y
91	212	1						Y
92	229	1						Y
93	258	1						Y
94	261	1						Y
95	277	1						Y
96	296	1						Y
97	353	1						Y
98	359	1						Y
99	375	1						Y
100	389	1			pfam01028 Topoisomerase_I	8.00E-07		Y

BlastX performed with Blosum62 matrix and an E-value cutoff of 0.1 against the non-redundant (NR) protein database of NCBI.

Indicates significance of match to NR or CDD sequence of previous column.

RPSblast performed against the conserved domains database (CDD) of NCBI containing all PFAM and Smart motifs.

⁴

Y, the sequence is a newly found Ixodes scapularis sequence.

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