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Table 1.

Thirty most abundant cDNA clusters from an adult female Ixodes scapularis salivary gland library

Cluster number	Number of sequences	Best BlastX match to NR database^¹	E-value^²	Species of NR match	Best Rpsblast to CDD database^³	E-value^²	SignalP^⁴
1	44	gi\|15428308\| 14 kDa salivary...	7E-34	Ixodes scapularis	pfam02414 Borrelia_orfA	1E-09	SIG
2	41				pfam02326 YMF19	0.0005	SIG
3	34	gi\|5835682\|gi\|5835682\|ref\|NP_008498.1\|CO	6E-83	Ixodes hexagonus	pfam00115 COX1	1E-90	NoORF
4	20	gi\|15428348\| Salp10 [Ixodes s...	5E-34	Ixodes scapularis	pfam00014 Kunitz_BPTI	0.001	SIG
5	18				pfam01028 Topoisomerase_I	0.0003	SIG
6	14						NoORF
7	14	gi\|124810\|IP52_ANESU PROTEASE INHIBITOR	0.01	Anemonia sulcata	Smart smart00131 KU	1E-06	SIG
8	11						NoORF
9	9	gi\|15428310\| 25 kDa salivary...	1E-105	Ixodes scapularis			SIG
10	8	gi\|5835692\|gi\|5835692\|ref\|NP_008508.1\|CY	2E-73	Ixodes hexagonus	pfam00033 cytochrome_b_N	1E-38	NoORF
11	8	gi\|5835683\|gi\|5835683\|ref\|NP_008499.1\|CO	6E-37	Ixodes hexagonus	pfam00116 COX2	5E-26	NoORF
12	8	gi\|5835693\|gi\|5835693\|ref\|NP_008509.1\|ND	1E-50	Ixodes hexagonus	pfam00146 NADHdh	2E-52	NoORF
13	8	gi\|7302028\| CG1746 gene product	7E-17	Drosophila melanogaster	pfam00137 ATP-synt_C	8E-20	ANCH
14	7						ANCH
15	7						NOSIG
16	7				Smart smart00131 KU	4E-05	ANCH
17	6	gi\|3881447\| contains similarity to Pfam	0.018		Smart smart00131 KU	0.0001	NoORF
18	6				pfam02098 His_binding	0.0007	SIG
19	5	gi\|3451024\| cytochrome oxidase III	2E-25	Ixodes pacificus	pfam00510 COX3	1E-48	NoORF
20	5	gi\|15428348\| Salp10 [Ixodes s...	9E-14	Ixodes scapularis			SIG
21	4	gi\|7861934\| Ke3 [Danio rerio]	2E-37	Danio rerio	pfam00416 Ribosomal_S13	6E-22	NOSIG
22	4	gi\|8072217\| Dscam [Drosophila...	0.056	Drosophila melanogaster			SIG
23	4						SIG
24	3				Smart smart00131 KU	0.007	SIG
25	3				pfam01943 Polysacc_synt	4E-08	NoORF
26	3	gi\|7506657\|gi\|7506657\|pir‖T32060	0.00001	Caenorhabditis elegans	Smart smart00131 KU	2E-09	NoORF
27	3	gi\|15428300\| 20 kDa salivary...	3E-62	Ixodes scapularis			NoORF
28	3				pfam01490 Aa_trans	0.01	NoORF
29	3				pfam01391 Collagen	0.0007	SIG
30	3				pfam00014 Kunitz_BPTI	0.009	SIG

Cluster number	Number of sequences	Best BlastX match to NR database^¹	E-value^²	Species of NR match	Best Rpsblast to CDD database^³	E-value^²	SignalP^⁴
1	44	gi\|15428308\| 14 kDa salivary...	7E-34	Ixodes scapularis	pfam02414 Borrelia_orfA	1E-09	SIG
2	41				pfam02326 YMF19	0.0005	SIG
3	34	gi\|5835682\|gi\|5835682\|ref\|NP_008498.1\|CO	6E-83	Ixodes hexagonus	pfam00115 COX1	1E-90	NoORF
4	20	gi\|15428348\| Salp10 [Ixodes s...	5E-34	Ixodes scapularis	pfam00014 Kunitz_BPTI	0.001	SIG
5	18				pfam01028 Topoisomerase_I	0.0003	SIG
6	14						NoORF
7	14	gi\|124810\|IP52_ANESU PROTEASE INHIBITOR	0.01	Anemonia sulcata	Smart smart00131 KU	1E-06	SIG
8	11						NoORF
9	9	gi\|15428310\| 25 kDa salivary...	1E-105	Ixodes scapularis			SIG
10	8	gi\|5835692\|gi\|5835692\|ref\|NP_008508.1\|CY	2E-73	Ixodes hexagonus	pfam00033 cytochrome_b_N	1E-38	NoORF
11	8	gi\|5835683\|gi\|5835683\|ref\|NP_008499.1\|CO	6E-37	Ixodes hexagonus	pfam00116 COX2	5E-26	NoORF
12	8	gi\|5835693\|gi\|5835693\|ref\|NP_008509.1\|ND	1E-50	Ixodes hexagonus	pfam00146 NADHdh	2E-52	NoORF
13	8	gi\|7302028\| CG1746 gene product	7E-17	Drosophila melanogaster	pfam00137 ATP-synt_C	8E-20	ANCH
14	7						ANCH
15	7						NOSIG
16	7				Smart smart00131 KU	4E-05	ANCH
17	6	gi\|3881447\| contains similarity to Pfam	0.018		Smart smart00131 KU	0.0001	NoORF
18	6				pfam02098 His_binding	0.0007	SIG
19	5	gi\|3451024\| cytochrome oxidase III	2E-25	Ixodes pacificus	pfam00510 COX3	1E-48	NoORF
20	5	gi\|15428348\| Salp10 [Ixodes s...	9E-14	Ixodes scapularis			SIG
21	4	gi\|7861934\| Ke3 [Danio rerio]	2E-37	Danio rerio	pfam00416 Ribosomal_S13	6E-22	NOSIG
22	4	gi\|8072217\| Dscam [Drosophila...	0.056	Drosophila melanogaster			SIG
23	4						SIG
24	3				Smart smart00131 KU	0.007	SIG
25	3				pfam01943 Polysacc_synt	4E-08	NoORF
26	3	gi\|7506657\|gi\|7506657\|pir‖T32060	0.00001	Caenorhabditis elegans	Smart smart00131 KU	2E-09	NoORF
27	3	gi\|15428300\| 20 kDa salivary...	3E-62	Ixodes scapularis			NoORF
28	3				pfam01490 Aa_trans	0.01	NoORF
29	3				pfam01391 Collagen	0.0007	SIG
30	3				pfam00014 Kunitz_BPTI	0.009	SIG

SIG, secretory signal sequence found; NoOrf, no suitable frame or start methionine was found; NoSIG, no secretory signal is found; ANCH, membrane anchor sequence found.

BlastX performed with Blosum62 matrix and an E-value cutoff of 0.1 against the non-redundant (NR) protein database of NCBI.

Indicates significance of match to NR or CDD sequence of previous column.

RPSblast performed against the conserved domains database (CDD) of NCBI containing all PFAM and Smart motifs.

⁴

Combined results from all sequences after submission of best open reading frame to the SignalP server (Ref^***)

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