Electrophysiological variables of pondwater- and high-salinity-adapted leech dorsal integuments
| . | APW . | HSW . |
|---|---|---|
| Control conditions | ||
| Vinit (m V) | 17.5±2.4 | 8.4±1.5* |
| VT (m V) | 32.7±3.9 | 16.2±2.5* |
| RT (Ω cm2) | 2038±346 | 1701±176 |
| Isc (μA cm-2) | 18.96±1.76 | 9.56±1.1* |
| Iami (μA cm-2) | 4.34±0.68 | 3.12±0.48 |
| INa (μA cm-2) | 7.66±0.82 | 4.6±0.54* |
| Apical Ca2+-free conditions | ||
| RT (Ω cm2) | 1735±179 | 1706±109 |
| Isc (μA cm-2) | 25.88±1.82† | 22.66±1.92† |
| Iami (μA cm-2) | 13.54±1.36† | 10.16±1.72† |
| INa (μA cm-2) | 19.44±1.1† | 15.96±1.62† |
| . | APW . | HSW . |
|---|---|---|
| Control conditions | ||
| Vinit (m V) | 17.5±2.4 | 8.4±1.5* |
| VT (m V) | 32.7±3.9 | 16.2±2.5* |
| RT (Ω cm2) | 2038±346 | 1701±176 |
| Isc (μA cm-2) | 18.96±1.76 | 9.56±1.1* |
| Iami (μA cm-2) | 4.34±0.68 | 3.12±0.48 |
| INa (μA cm-2) | 7.66±0.82 | 4.6±0.54* |
| Apical Ca2+-free conditions | ||
| RT (Ω cm2) | 1735±179 | 1706±109 |
| Isc (μA cm-2) | 25.88±1.82† | 22.66±1.92† |
| Iami (μA cm-2) | 13.54±1.36† | 10.16±1.72† |
| INa (μA cm-2) | 19.44±1.1† | 15.96±1.62† |
APW, artificial pondwater; HSW, high-salinity water.
Vinit, initial transepithelial potential; VT, transepithelial potential; RT,transepithelial resistance; Isc, short-circuit current; Iami, amiloride-sensitive current; INa, transepithelial Na+ current.
Values are means ± S.E.M., N=16.
Values of HSW-adapted integuments significantly different from values of APW-adapted integuments, P<0.05.
Ca2+-free values significantly different from control values, P<0.05.