The Company of Biologists

Table 2.

Anopheles gambiae secretory cDNAs

Clus^¹	R^²	GenBank match^³	E-value^⁴	Gi numb^⁵	PFAM match^⁶	E-value^⁷	Org^⁸	N^⁹	Function^¹⁰	F^¹¹	Clone^¹²	FASTA^¹³	CLUSTAL^¹⁴
6	33	gi\|2114497\| 30 kDa salivary gland	7.00E-07	gi\|2114497	pfam01127 Sdh_cyt	7.00E-07	Aedes aegypti	Y	30 kDa allergen family	S	SIG	AGN10E60-6clu.txt	AGN10E60-6aln.txt
58	2	gi\|159550\| amylase [Aedes aegypti]	1.00E-66	gi\|159550	Smart smart00632 Aamy_C	1.00E-14	Aedes aegypti	Y	Amylase	S	NoORF	AGN10E60-58clu.txt	AGN10E60-58aln.txt
105	1	gi\|2285788\| Multiprotein bridging fac...	5.00E-51	gi\|2285788	pfam01381 HTH_3	3.00E-06	Bombyx mori	Y	Angiogenesis function? —Similar to human EDF-1	S	NoORF	AGN10E60-105clu.txt
17	6	gi\|4127344\| cE5 protein [Anopheles	7.00E-20	gi\|4127344	pfam01028 Topoisomerase_I	1.00E-09	Anopheles gambiae	N	Anticlotting	S	SIG	AGN10E60-17clu.txt	AGN10E60-17aln.txt
14	8	gi\|8927462\| antigen 5 precur...	3.00E-36	gi\|8927462	Smart smart00198 SCP	9.00E-30	Glossina morsitans morsitans	Y	Antigen 5 family	S	SIG	AGN10E60-14clu.txt	AGN10E60-14aln.txt
69	1	gi\|7292977\| CG9400 gene product	1.00E-18	gi\|7292977	Smart smart00198 SCP	2.00E-20	Drosophila melanogaster	Y	Antigen 5 protein	S	SIG	AGN10E60-69clu.txt
210	1	gi\|4887102\| antigen 5-relate...	3.00E-08	gi\|4887102	Smart smart00198 SCP	9.00E-08	Lutzomyia longipalpis	Y	Antigen 5 protein	S	NOSIG	AGN10E60-210clu.txt
183	1	gi\|7299219\| Crc gene product	5.00E-87	gi\|7299219	pfam00262 calreticulin	3.00E-70	Drosophila melanogaster	Y	Calreticulin	S	SIG	AGN10E60-183clu.txt
15	7	gi\|159559\| D7 protein [Aedes aegypti]	4.00E-23	gi\|159559	No matches found		Aedes aegypti	Y	D7 protein	S	SIG	AGN10E60-15clu.txt	AGN10E60-15aln.txt
8	23	gi\|4538887\| D7-related 1 protein [Ano...	9.00E-82	gi\|4538887	pfam01604 7tm_5	9.00E-05	Anopheles gambiae	N	D7r1 protein	S	SIG	AGN10E60-8clu.txt	AGN10E60-8aln.txt
208	1	gi\|4538887\| D7-related 1 protein [Ano...	7.00E-07	gi\|4538887	No matches found		Anopheles gambiae	Y	D7r1 protein new member	S	NoORF	AGN10E60-208clu.txt
88	1	gi\|4538889\| D7-related 2 protein [Ano...	4.00E-05	gi\|4538889	No matches found		Anopheles gambiae	Y	D7r2 family — New member	S	NoORF	AGN10E60-88clu.txt
1	49	gi\|4538889\| D7-related 2 protein [Ano...	8.00E-77	gi\|4538889	pfam02326 YMF19	0.001	Anopheles gambiae	N	D7r2 protein	S	SIG	AGN10E60-1clu.txt	AGN10E60-1aln.txt
2	47	gi\|4538891\| D7-related 3 protein [Ano...	4.00E-94	gi\|4538891	pfam01028 Topoisomerase_I	3.00E-05	Anopheles gambiae	N	D7r3 protein	S	SIG	AGN10E60-2clu.txt	AGN10E60-2aln.txt
11	12	gi\|13537670\| D7r4 protein [Anopheles...	3.00E-94	gi\|13537670	pfam01598 Sterol_desat	0.007	Anopheles gambiae	N	D7r4 protein	S	SIG	AGN10E60-11clu.txt	AGN10E60-11aln.txt
26	3	gi\|13537670\| D7r4 protein [Anopheles...	4.00E-17	gi\|13537670	No matches found		Anopheles gambiae	Y	D7r protein new member	S	SIG	AGN10E60-26clu.txt	AGN10E60-26aln.txt
56	2	gi\|13537664\| gSG1-like 2 protein [Ano...	1.00E-126	gi\|13537664	pfam01500 Keratin_B2	5.00E-04	Anopheles gambiae	N	gSG1-like 2 protein	S	NoORF	AGN10E60-56clu.txt	AGN10E60-56aln.txt
5	33	gi\|13537660\| gSG2-like protein [Anoph...	3.00E-30	gi\|13537660	No matches found		Anopheles gambiae	N	gSG2 protein	S	SIG	AGN10E60-5clu.txt	AGN10E60-5aln.txt
13	9	gi\|13537662\| gSG5 protein [Anopheles...	6.00E-93	gi\|13537662	Smart smart00052 DUF2	0.005	Anopheles gambiae	N	gSG5 protein	S	SIG	AGN10E60-13clu.txt	AGN10E60-13aln.txt
9	20	gi\|13537666\| gSG6 protein [Anopheles...	3.00E-55	gi\|13537666	pfam01688 Herpes_gI	0.002	Anopheles gambiae	N	gSG6 protein	S	SIG	AGN10E60-9clu.txt	AGN10E60-9aln.txt
12	10	gi\|13537668\| gSG7 protein [Anopheles...	3.00E-77	gi\|13537668	No matches found		Anopheles gambiae	N	gSG7 protein	S	SIG	AGN10E60-12clu.txt	AGN10E60-12aln.txt
19	6	gi\|13537668\| gSG7 protein [Anopheles...	3.00E-09	gi\|13537668	No matches found		Anopheles gambiae	Y	gSG7 protein — new member?	S	SIG	AGN10E60-19clu.txt	AGN10E60-19aln.txt
22	5	gi\|13537672\| hypothetical protein [An...	2.00E-74	gi\|13537672	pfam00710 Asparaginase	6.00E-04	Anopheles gambiae	N	HP gi\|13537672	S	ANCH	AGN10E60-22clu.txt	AGN10E60-22aln.txt
7	31	gi\|4127307\| hypothetical protein	7.00E-05	gi\|4127307	pfam01028 Topoisomerase_I	0.002	Anopheles gambiae	N	HP gi\|4127307	S	SIG	AGN10E60-7clu.txt	AGN10E60-7aln.txt
10	13	gi\|894206\| lysozyme [Anopheles gambiae]	2.00E-71	gi\|894206	Smart smart00263 LYZ1	1.00E-52	Anopheles gambiae	N	Lyzozyme	S	SIG	AGN10E60-10clu.txt	AGN10E60-10aln.txt
34	2	No matches found	pfam01456 Tryp_mucin		2.00E-07			Y	Mucin	S	SIG	AGN10E60-34clu.txt	AGN10E60-34aln.txt
16	6	gi\|4582528\| putative 5′-nucleotidase...	1.00E-129	gi\|4582528	pfam02872 5_nucleotidaseC	1.00E-49	Anopheles gambiae	N	Putative 5′-nuc truncated clone	S	NoORF	AGN10E60-16clu.txt	AGN10E60-16aln.txt
46	2	gi\|4582528\| putative 5′-nucleotidase...	3.00E-73	gi\|4582528	pfam01009 5_nucleotidase	1.00E-24	Anopheles gambiae	N	Putative 5′-nucleotidase	S	SIG	AGN10E60-46clu.txt	AGN10E60-46aln.txt
20	6	gi\|4582526\| putative apyrase [Anophel...	2.00E-41	gi\|4582526	pfam02872 5_nucleotidaseC	2.00E-06	Anopheles gambiae	N	Putative apyrase — truncated clone	S	NOSIG	AGN10E60-20clu.txt	AGN10E60-20aln.txt
128	1	gi\|7293955\| CG7484 gene product	2.00E-31	gi\|7293955	No matches found		Drosophila melanogaster	Y	Selenoprotein precursor	S	SIG	AGN10E60-128clu.txt
212	1	gi\|4210615\| SG1 protein [Anopheles ga...	4.00E-07	gi\|4210615	No matches found		Anopheles gambiae	Y	SG1 protein — New member?	S	NOSIG	AGN10E60-212clu.txt
37	2	gi\|4210615\| SG1 protein [Anopheles ga...	2.00E-10	gi\|4210615	pfam02122 Luteo_ORF2	3.00E-04	Anopheles gambiae	Y	SG1 protein — New member?	S	NOSIG	AGN10E60-37clu.txt	AGN10E60-37aln.txt
21	5	gi\|4210617\| SG2 protein [Anopheles ga...	1.00E-23	gi\|4210617	pfam01028 Topoisomerase_I	2.00E-09	Anopheles gambiae	N	SG2	S	SIG	AGN10E60-21clu.txt	AGN10E60-21aln.txt
4	38	No matches found			No matches found			Y	Unknown protein	S	SIG	AGN10E60-4clu.txt	AGN10E60-4aln.txt
27	3	No matches found			No matches found			Y	Unknown protein	S	SIG	AGN10E60-27clu.txt	AGN10E60-27aln.txt
68	1	No matches found			No matches found			Y	Unknown protein	S	SIG	AGN10E60-68clu.txt
83	1	No matches found			No matches found			Y	Unknown protein	S	SIG	AGN10E60-83clu.txt
90	1	No matches found			pfam02326 YMF19	0.005		Y	Unknown protein	S	SIG	AGN10E60-90clu.txt
93	1	No matches found			No matches found			Y	Unknown protein	S	SIG	AGN10E60-93clu.txt
134	1	No matches found			No matches found			Y	Unknown protein	S	SIG	AGN10E60-134clu.txt
139	1	No matches found			pfam01604 7tm_5	0.007		Y	Unknown protein	S	SIG	AGN10E60-139clu.txt
157	1	No matches found			No matches found			Y	Unknown protein	S	SIG	AGN10E60-157clu.txt
162	1	No matches found			No matches found			Y	Unknown protein	S	SIG	AGN10E60-162clu.txt
188	1	No matches found			No matches found			Y	Unknown protein	S	SIG	AGN10E60-188clu.txt
29	3	No matches found			pfam01490 Aa_trans	0.002		Y	Unknown protein	S	SIG	AGN10E60-29clu.txt	AGN10E60-29aln.txt

Clus^¹	R^²	GenBank match^³	E-value^⁴	Gi numb^⁵	PFAM match^⁶	E-value^⁷	Org^⁸	N^⁹	Function^¹⁰	F^¹¹	Clone^¹²	FASTA^¹³	CLUSTAL^¹⁴
6	33	gi\|2114497\| 30 kDa salivary gland	7.00E-07	gi\|2114497	pfam01127 Sdh_cyt	7.00E-07	Aedes aegypti	Y	30 kDa allergen family	S	SIG	AGN10E60-6clu.txt	AGN10E60-6aln.txt
58	2	gi\|159550\| amylase [Aedes aegypti]	1.00E-66	gi\|159550	Smart smart00632 Aamy_C	1.00E-14	Aedes aegypti	Y	Amylase	S	NoORF	AGN10E60-58clu.txt	AGN10E60-58aln.txt
105	1	gi\|2285788\| Multiprotein bridging fac...	5.00E-51	gi\|2285788	pfam01381 HTH_3	3.00E-06	Bombyx mori	Y	Angiogenesis function? —Similar to human EDF-1	S	NoORF	AGN10E60-105clu.txt
17	6	gi\|4127344\| cE5 protein [Anopheles	7.00E-20	gi\|4127344	pfam01028 Topoisomerase_I	1.00E-09	Anopheles gambiae	N	Anticlotting	S	SIG	AGN10E60-17clu.txt	AGN10E60-17aln.txt
14	8	gi\|8927462\| antigen 5 precur...	3.00E-36	gi\|8927462	Smart smart00198 SCP	9.00E-30	Glossina morsitans morsitans	Y	Antigen 5 family	S	SIG	AGN10E60-14clu.txt	AGN10E60-14aln.txt
69	1	gi\|7292977\| CG9400 gene product	1.00E-18	gi\|7292977	Smart smart00198 SCP	2.00E-20	Drosophila melanogaster	Y	Antigen 5 protein	S	SIG	AGN10E60-69clu.txt
210	1	gi\|4887102\| antigen 5-relate...	3.00E-08	gi\|4887102	Smart smart00198 SCP	9.00E-08	Lutzomyia longipalpis	Y	Antigen 5 protein	S	NOSIG	AGN10E60-210clu.txt
183	1	gi\|7299219\| Crc gene product	5.00E-87	gi\|7299219	pfam00262 calreticulin	3.00E-70	Drosophila melanogaster	Y	Calreticulin	S	SIG	AGN10E60-183clu.txt
15	7	gi\|159559\| D7 protein [Aedes aegypti]	4.00E-23	gi\|159559	No matches found		Aedes aegypti	Y	D7 protein	S	SIG	AGN10E60-15clu.txt	AGN10E60-15aln.txt
8	23	gi\|4538887\| D7-related 1 protein [Ano...	9.00E-82	gi\|4538887	pfam01604 7tm_5	9.00E-05	Anopheles gambiae	N	D7r1 protein	S	SIG	AGN10E60-8clu.txt	AGN10E60-8aln.txt
208	1	gi\|4538887\| D7-related 1 protein [Ano...	7.00E-07	gi\|4538887	No matches found		Anopheles gambiae	Y	D7r1 protein new member	S	NoORF	AGN10E60-208clu.txt
88	1	gi\|4538889\| D7-related 2 protein [Ano...	4.00E-05	gi\|4538889	No matches found		Anopheles gambiae	Y	D7r2 family — New member	S	NoORF	AGN10E60-88clu.txt
1	49	gi\|4538889\| D7-related 2 protein [Ano...	8.00E-77	gi\|4538889	pfam02326 YMF19	0.001	Anopheles gambiae	N	D7r2 protein	S	SIG	AGN10E60-1clu.txt	AGN10E60-1aln.txt
2	47	gi\|4538891\| D7-related 3 protein [Ano...	4.00E-94	gi\|4538891	pfam01028 Topoisomerase_I	3.00E-05	Anopheles gambiae	N	D7r3 protein	S	SIG	AGN10E60-2clu.txt	AGN10E60-2aln.txt
11	12	gi\|13537670\| D7r4 protein [Anopheles...	3.00E-94	gi\|13537670	pfam01598 Sterol_desat	0.007	Anopheles gambiae	N	D7r4 protein	S	SIG	AGN10E60-11clu.txt	AGN10E60-11aln.txt
26	3	gi\|13537670\| D7r4 protein [Anopheles...	4.00E-17	gi\|13537670	No matches found		Anopheles gambiae	Y	D7r protein new member	S	SIG	AGN10E60-26clu.txt	AGN10E60-26aln.txt
56	2	gi\|13537664\| gSG1-like 2 protein [Ano...	1.00E-126	gi\|13537664	pfam01500 Keratin_B2	5.00E-04	Anopheles gambiae	N	gSG1-like 2 protein	S	NoORF	AGN10E60-56clu.txt	AGN10E60-56aln.txt
5	33	gi\|13537660\| gSG2-like protein [Anoph...	3.00E-30	gi\|13537660	No matches found		Anopheles gambiae	N	gSG2 protein	S	SIG	AGN10E60-5clu.txt	AGN10E60-5aln.txt
13	9	gi\|13537662\| gSG5 protein [Anopheles...	6.00E-93	gi\|13537662	Smart smart00052 DUF2	0.005	Anopheles gambiae	N	gSG5 protein	S	SIG	AGN10E60-13clu.txt	AGN10E60-13aln.txt
9	20	gi\|13537666\| gSG6 protein [Anopheles...	3.00E-55	gi\|13537666	pfam01688 Herpes_gI	0.002	Anopheles gambiae	N	gSG6 protein	S	SIG	AGN10E60-9clu.txt	AGN10E60-9aln.txt
12	10	gi\|13537668\| gSG7 protein [Anopheles...	3.00E-77	gi\|13537668	No matches found		Anopheles gambiae	N	gSG7 protein	S	SIG	AGN10E60-12clu.txt	AGN10E60-12aln.txt
19	6	gi\|13537668\| gSG7 protein [Anopheles...	3.00E-09	gi\|13537668	No matches found		Anopheles gambiae	Y	gSG7 protein — new member?	S	SIG	AGN10E60-19clu.txt	AGN10E60-19aln.txt
22	5	gi\|13537672\| hypothetical protein [An...	2.00E-74	gi\|13537672	pfam00710 Asparaginase	6.00E-04	Anopheles gambiae	N	HP gi\|13537672	S	ANCH	AGN10E60-22clu.txt	AGN10E60-22aln.txt
7	31	gi\|4127307\| hypothetical protein	7.00E-05	gi\|4127307	pfam01028 Topoisomerase_I	0.002	Anopheles gambiae	N	HP gi\|4127307	S	SIG	AGN10E60-7clu.txt	AGN10E60-7aln.txt
10	13	gi\|894206\| lysozyme [Anopheles gambiae]	2.00E-71	gi\|894206	Smart smart00263 LYZ1	1.00E-52	Anopheles gambiae	N	Lyzozyme	S	SIG	AGN10E60-10clu.txt	AGN10E60-10aln.txt
34	2	No matches found	pfam01456 Tryp_mucin		2.00E-07			Y	Mucin	S	SIG	AGN10E60-34clu.txt	AGN10E60-34aln.txt
16	6	gi\|4582528\| putative 5′-nucleotidase...	1.00E-129	gi\|4582528	pfam02872 5_nucleotidaseC	1.00E-49	Anopheles gambiae	N	Putative 5′-nuc truncated clone	S	NoORF	AGN10E60-16clu.txt	AGN10E60-16aln.txt
46	2	gi\|4582528\| putative 5′-nucleotidase...	3.00E-73	gi\|4582528	pfam01009 5_nucleotidase	1.00E-24	Anopheles gambiae	N	Putative 5′-nucleotidase	S	SIG	AGN10E60-46clu.txt	AGN10E60-46aln.txt
20	6	gi\|4582526\| putative apyrase [Anophel...	2.00E-41	gi\|4582526	pfam02872 5_nucleotidaseC	2.00E-06	Anopheles gambiae	N	Putative apyrase — truncated clone	S	NOSIG	AGN10E60-20clu.txt	AGN10E60-20aln.txt
128	1	gi\|7293955\| CG7484 gene product	2.00E-31	gi\|7293955	No matches found		Drosophila melanogaster	Y	Selenoprotein precursor	S	SIG	AGN10E60-128clu.txt
212	1	gi\|4210615\| SG1 protein [Anopheles ga...	4.00E-07	gi\|4210615	No matches found		Anopheles gambiae	Y	SG1 protein — New member?	S	NOSIG	AGN10E60-212clu.txt
37	2	gi\|4210615\| SG1 protein [Anopheles ga...	2.00E-10	gi\|4210615	pfam02122 Luteo_ORF2	3.00E-04	Anopheles gambiae	Y	SG1 protein — New member?	S	NOSIG	AGN10E60-37clu.txt	AGN10E60-37aln.txt
21	5	gi\|4210617\| SG2 protein [Anopheles ga...	1.00E-23	gi\|4210617	pfam01028 Topoisomerase_I	2.00E-09	Anopheles gambiae	N	SG2	S	SIG	AGN10E60-21clu.txt	AGN10E60-21aln.txt
4	38	No matches found			No matches found			Y	Unknown protein	S	SIG	AGN10E60-4clu.txt	AGN10E60-4aln.txt
27	3	No matches found			No matches found			Y	Unknown protein	S	SIG	AGN10E60-27clu.txt	AGN10E60-27aln.txt
68	1	No matches found			No matches found			Y	Unknown protein	S	SIG	AGN10E60-68clu.txt
83	1	No matches found			No matches found			Y	Unknown protein	S	SIG	AGN10E60-83clu.txt
90	1	No matches found			pfam02326 YMF19	0.005		Y	Unknown protein	S	SIG	AGN10E60-90clu.txt
93	1	No matches found			No matches found			Y	Unknown protein	S	SIG	AGN10E60-93clu.txt
134	1	No matches found			No matches found			Y	Unknown protein	S	SIG	AGN10E60-134clu.txt
139	1	No matches found			pfam01604 7tm_5	0.007		Y	Unknown protein	S	SIG	AGN10E60-139clu.txt
157	1	No matches found			No matches found			Y	Unknown protein	S	SIG	AGN10E60-157clu.txt
162	1	No matches found			No matches found			Y	Unknown protein	S	SIG	AGN10E60-162clu.txt
188	1	No matches found			No matches found			Y	Unknown protein	S	SIG	AGN10E60-188clu.txt
29	3	No matches found			pfam01490 Aa_trans	0.002		Y	Unknown protein	S	SIG	AGN10E60-29clu.txt	AGN10E60-29aln.txt

Clus, cluster number.

R, number of sequences of a given custer (R, representation).

GenBank match, best match to the GenBank database.

⁴

E-value, indicates significance of match to NR sequence of previous column.

⁵

Gi numb, GenBank accession number.

⁶

PFAM match, best match to the PFAM database.

⁷

E-value, indicates significance of match to CDD sequence of previous column.

⁸

Org, organism where a match was found.

⁹

N, Novel (Y, yes; N, no).

¹⁰

Function, putative function or biological property.

¹¹

F, Function; S, secretory

¹²

Clone: NOSIG, no signal peptide detected by SignalP server; No ORF, absence of a open-reading frame; ANCH, anchor protein.

¹³

FASTA, FASTA-formatted sequences.

¹⁴

CLUSTAL, clustal alignment for two or more sequences.

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