Response of ligatured flies to bursicon or cyclic AMP
| . | % Tanning . | . | |
|---|---|---|---|
| Solution . | Wild type . | rk4/rk4 . | |
| Saline | 0 (10) | 0 (10) | |
| Bursicon | 100 (10) | 0 (16) | |
| cAMP | 100 (6) | 92 (13) | |
| . | % Tanning . | . | |
|---|---|---|---|
| Solution . | Wild type . | rk4/rk4 . | |
| Saline | 0 (10) | 0 (10) | |
| Bursicon | 100 (10) | 0 (16) | |
| cAMP | 100 (6) | 92 (13) | |
Saline solutions containing either bursicon or 8-Br-cAMP (100-200 pmoles)were injected into neck-ligatured wild-type or mutant(rk4/rk4) flies.
% Tanning, the percentage of animals that responded to the injection by melanizing their cuticle; the number of animals injected is given in parentheses.
Bursicon was extracted from Sarcophaga bullata nervous system and approximately 0.13 nervous system equivalents were injected into each fly.