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Table 1.

Production of predominant hydrocarbons in mature D. melanogaster flies raised at various temperatures

Hydrocarbons produced (ng)
FlyHydrocarbonConstant 20°C25→20°C20→25°CConstant 25°CK—W test
Cs males 7-T 1043±45a 1052±82a 846±37b 878±60a,b P<0.05 
 7-P 106±9a 112±9a 225±15b 154±10b P<0.001 
 SumCHs 1584±58 1628±105 1600±65 1550±87 N.S. 
Cs females 7-T 159±14a 149±8a 71±6b 56±6b P<0.001 
 7-P 172±12a 151±8a 116±7b 92±7c P<0.001 
 7,11HD 534±36a 563±40a 373±17b 417±30a,b P<0.01 
 7,11ND 217±16a 227±28a,b 295±17b 306±17b P<0.05 
 SumCHs 1986±92a,c 2049±97a 1724±78b 1702±104b,c P<0.05 
Tai males 7-T 264±14a 290±24a 74±8a,b 102±7b P<0.001 
 7-P 1002±54 1027±86 908±62 848±70 N.S. 
 SumCHs 1885±88a 2014±151a 1639±102a,b 1540±121b P<0.05 
Tai females 7,11HD 134±7a 180±18a 75±6b 92±10b P<0.001 
 5,9HD 997±44 975±69 817±71 870±41 N.S. 
 7,11ND 168±11 173±17 149±15 154±15 N.S. 
 SumCHs 1661±69 1748±127 1450±106 1542±92 N.S. 
Hydrocarbons produced (ng)
FlyHydrocarbonConstant 20°C25→20°C20→25°CConstant 25°CK—W test
Cs males 7-T 1043±45a 1052±82a 846±37b 878±60a,b P<0.05 
 7-P 106±9a 112±9a 225±15b 154±10b P<0.001 
 SumCHs 1584±58 1628±105 1600±65 1550±87 N.S. 
Cs females 7-T 159±14a 149±8a 71±6b 56±6b P<0.001 
 7-P 172±12a 151±8a 116±7b 92±7c P<0.001 
 7,11HD 534±36a 563±40a 373±17b 417±30a,b P<0.01 
 7,11ND 217±16a 227±28a,b 295±17b 306±17b P<0.05 
 SumCHs 1986±92a,c 2049±97a 1724±78b 1702±104b,c P<0.05 
Tai males 7-T 264±14a 290±24a 74±8a,b 102±7b P<0.001 
 7-P 1002±54 1027±86 908±62 848±70 N.S. 
 SumCHs 1885±88a 2014±151a 1639±102a,b 1540±121b P<0.05 
Tai females 7,11HD 134±7a 180±18a 75±6b 92±10b P<0.001 
 5,9HD 997±44 975±69 817±71 870±41 N.S. 
 7,11ND 168±11 173±17 149±15 154±15 N.S. 
 SumCHs 1661±69 1748±127 1450±106 1542±92 N.S. 

Values are means of absolute amounts (ng) ± S.E.M.

Flies of two wild-type strains, Canton-S (Cs) and Tai, were either raised at a constant temperature (20°C or 25°C) throughout development, or shifted immediately after adult eclosion from 25°C to 20°C(25→20°C) or from 20°C to 25°C (20→25°C).

Hydrocarbons were extracted from 4- or 6-day-old flies (see Materials and methods). Hydrocarbons shown are cis 7-tricosene (7-T; 23C); cis 7-pentacosene (7-P; 25C); cis, cis7, 11-heptacosadiene(7,11HD); cis, cis5,9-heptacosadiene (5,9HD); cis,cis7,11-nonacosadiene (7,11ND), and the sum of all detected hydrocarbons(SumCHs). 5,9HD was not used for the tests because we could not separate 5,9HD and 27BR in our gas chromatograph conditions.

For each genotype (sex and strain), the result of Kruskall—Wallis(KW) tests that were used to compare experimental groups are shown on the right. When the test was significant, a post-hoc Mann—Whitney U test was used to compare groups in pairs. Five comparisons were performed: between constant temperatures and between each constant temperature and the shift condition. (Only the two shifting conditions were not compared). Superscript letters indicate significant differences between conditions(N=8-10); N.S., not significant.

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