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Table 1.

Production of predominant hydrocarbons in mature D. melanogaster flies raised at various temperatures

		Hydrocarbons produced (ng)
Fly	Hydrocarbon	Constant 20°C	25→20°C	20→25°C	Constant 25°C	K—W test
Cs males	7-T	1043±45^a	1052±82^a	846±37^b	878±60^a,b	P<0.05
	7-P	106±9^a	112±9^a	225±15^b	154±10^b	P<0.001
	SumCHs	1584±58	1628±105	1600±65	1550±87	N.S.
Cs females	7-T	159±14^a	149±8^a	71±6^b	56±6^b	P<0.001
	7-P	172±12^a	151±8^a	116±7^b	92±7^c	P<0.001
	7,11HD	534±36^a	563±40^a	373±17^b	417±30^a,b	P<0.01
	7,11ND	217±16^a	227±28^a,b	295±17^b	306±17^b	P<0.05
	SumCHs	1986±92^a,c	2049±97^a	1724±78^b	1702±104^b,c	P<0.05
Tai males	7-T	264±14^a	290±24^a	74±8^a,b	102±7^b	P<0.001
	7-P	1002±54	1027±86	908±62	848±70	N.S.
	SumCHs	1885±88^a	2014±151^a	1639±102^a,b	1540±121^b	P<0.05
Tai females	7,11HD	134±7^a	180±18^a	75±6^b	92±10^b	P<0.001
	5,9HD	997±44	975±69	817±71	870±41	N.S.
	7,11ND	168±11	173±17	149±15	154±15	N.S.
	SumCHs	1661±69	1748±127	1450±106	1542±92	N.S.

		Hydrocarbons produced (ng)
Fly	Hydrocarbon	Constant 20°C	25→20°C	20→25°C	Constant 25°C	K—W test
Cs males	7-T	1043±45^a	1052±82^a	846±37^b	878±60^a,b	P<0.05
	7-P	106±9^a	112±9^a	225±15^b	154±10^b	P<0.001
	SumCHs	1584±58	1628±105	1600±65	1550±87	N.S.
Cs females	7-T	159±14^a	149±8^a	71±6^b	56±6^b	P<0.001
	7-P	172±12^a	151±8^a	116±7^b	92±7^c	P<0.001
	7,11HD	534±36^a	563±40^a	373±17^b	417±30^a,b	P<0.01
	7,11ND	217±16^a	227±28^a,b	295±17^b	306±17^b	P<0.05
	SumCHs	1986±92^a,c	2049±97^a	1724±78^b	1702±104^b,c	P<0.05
Tai males	7-T	264±14^a	290±24^a	74±8^a,b	102±7^b	P<0.001
	7-P	1002±54	1027±86	908±62	848±70	N.S.
	SumCHs	1885±88^a	2014±151^a	1639±102^a,b	1540±121^b	P<0.05
Tai females	7,11HD	134±7^a	180±18^a	75±6^b	92±10^b	P<0.001
	5,9HD	997±44	975±69	817±71	870±41	N.S.
	7,11ND	168±11	173±17	149±15	154±15	N.S.
	SumCHs	1661±69	1748±127	1450±106	1542±92	N.S.

Values are means of absolute amounts (ng) ± S.E.M.

Flies of two wild-type strains, Canton-S (Cs) and Tai, were either raised at a constant temperature (20°C or 25°C) throughout development, or shifted immediately after adult eclosion from 25°C to 20°C(25→20°C) or from 20°C to 25°C (20→25°C).

Hydrocarbons were extracted from 4- or 6-day-old flies (see Materials and methods). Hydrocarbons shown are cis 7-tricosene (7-T; 23C); cis 7-pentacosene (7-P; 25C); cis, cis7, 11-heptacosadiene(7,11HD); cis, cis5,9-heptacosadiene (5,9HD); cis,cis7,11-nonacosadiene (7,11ND), and the sum of all detected hydrocarbons(SumCHs). 5,9HD was not used for the tests because we could not separate 5,9HD and 27BR in our gas chromatograph conditions.

For each genotype (sex and strain), the result of Kruskall—Wallis(KW) tests that were used to compare experimental groups are shown on the right. When the test was significant, a post-hoc Mann—Whitney U test was used to compare groups in pairs. Five comparisons were performed: between constant temperatures and between each constant temperature and the shift condition. (Only the two shifting conditions were not compared). Superscript letters indicate significant differences between conditions(N=8-10); N.S., not significant.

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