Table 6.

Concentrations of free amino acids (FAA) and total free amino acids(TFAA) in liver of various tissues of B. sinensis after exposure to NH4Cl for 2 days

Muscle (μmol g-1)Liver (μmol g-1)Blood plasma (μmol ml-1)
FAAControl+NH4ClControl+NH4ClControl+NH4Cl
Ala 1.604±0.146 2.865±0.310(3)* 0.327±0.104 0.190±0.017 0.117±0.012 0.111±0.019 
Arg 0.726±0.065 0.572±0.032 0.046±0.004 0.045±0.007 0.016±0.006 0.013±0.002 
Asg 1.072±0.114 1.005±0.152 n.d n.d. 0.028±0.005 0.010±0.001* 
Asp 1.161±0.119 0.259±0.050* 0.539±0.139 0.188±0.037* 0.041±0.007(3) 0.021±0.004 
Cys n.d. n.d. n.d. n.d. 0.010±0.003 0.011±0.006 
Gln 2.639±0.247 8.118±0.067* n.d. 3.307±0.327(3) 0.079±0.023 0.280±0.045(3)* 
Glu 0.667±0.069 0.306±0.045* 3.803±0.013 6.984±0.233* 0.039±0.003(3) 0.040±0.002(3) 
Gly 14.36±1.85 16.61±2.54 2.503±0.474 1.669±0.231 0.497±0.086 0.401±0.033 
His 6.580±0.625 6.755±0.992 0.328±0.042 0.190±0.022* 0.130±0.016(3) 0.123±0.023(3) 
Ile 0.081±0.012 0.049±0.004 0.022±0.002(3) 0.021±0.005 0.074±0.012 0.020±0.010* 
Leu 0.144±0.018 0.097±0.008 0.042±0.003(3) 0.048±0.006(3) 0.131±0.020 0.061±0.009(3)* 
Lys 6.580±0.455 5.521±0.091 0.397±0.058 0.238±0.041 0.351±0.073 0.287±0.039 
Met n.d. n.d. n.d. n.d. 0.035±0.008 0.022±0.007(3) 
Phe 0.130±0.005 0.096±0.014* 0.055±0.006 0.035±0.003 0.069±0.004 0.024±0.005(3)* 
Pro 0.227±0.003 0.351±0.098(3) 0.129±0.024 0.121±0.019 0.090±0.056 0.019±0.004 
Ser 0.906±0.042 1.156±0.154 0.084±0.013 0.050±0.006 0.038±0.005 0.026±0.004 
Tau 16.70±1.46 18.30±2.44 17.00±1.12 14.17±0.42 0.387±0.074 0.321±0.041 
Thr 1.290±0.129 1.337±0.302 0.352±0.078(3) 0.485±0.082 0.451±0.078(3) 0.184±0.037(3)* 
Try 0.058±0.005 0.031±0.005* 0.042±0.008 0.021±0.004 0.060±0.003 0.027±0.003(3)* 
Tyr 0.064±0.004 0.053±0.005 0.055±0.006 0.058±0.005 0.036±0.002 0.019±0.005(3)* 
Val 0.145±0.016 0.100±0.013 0.054±0.017 0.032±0.007 0.118±0.016 0.099±0.025 
TFAA 55.12±4.00 69.69±4.29* 26.62±1.76 25.27±3.06(3) 2.786±0.310 2.267±0.241 
Muscle (μmol g-1)Liver (μmol g-1)Blood plasma (μmol ml-1)
FAAControl+NH4ClControl+NH4ClControl+NH4Cl
Ala 1.604±0.146 2.865±0.310(3)* 0.327±0.104 0.190±0.017 0.117±0.012 0.111±0.019 
Arg 0.726±0.065 0.572±0.032 0.046±0.004 0.045±0.007 0.016±0.006 0.013±0.002 
Asg 1.072±0.114 1.005±0.152 n.d n.d. 0.028±0.005 0.010±0.001* 
Asp 1.161±0.119 0.259±0.050* 0.539±0.139 0.188±0.037* 0.041±0.007(3) 0.021±0.004 
Cys n.d. n.d. n.d. n.d. 0.010±0.003 0.011±0.006 
Gln 2.639±0.247 8.118±0.067* n.d. 3.307±0.327(3) 0.079±0.023 0.280±0.045(3)* 
Glu 0.667±0.069 0.306±0.045* 3.803±0.013 6.984±0.233* 0.039±0.003(3) 0.040±0.002(3) 
Gly 14.36±1.85 16.61±2.54 2.503±0.474 1.669±0.231 0.497±0.086 0.401±0.033 
His 6.580±0.625 6.755±0.992 0.328±0.042 0.190±0.022* 0.130±0.016(3) 0.123±0.023(3) 
Ile 0.081±0.012 0.049±0.004 0.022±0.002(3) 0.021±0.005 0.074±0.012 0.020±0.010* 
Leu 0.144±0.018 0.097±0.008 0.042±0.003(3) 0.048±0.006(3) 0.131±0.020 0.061±0.009(3)* 
Lys 6.580±0.455 5.521±0.091 0.397±0.058 0.238±0.041 0.351±0.073 0.287±0.039 
Met n.d. n.d. n.d. n.d. 0.035±0.008 0.022±0.007(3) 
Phe 0.130±0.005 0.096±0.014* 0.055±0.006 0.035±0.003 0.069±0.004 0.024±0.005(3)* 
Pro 0.227±0.003 0.351±0.098(3) 0.129±0.024 0.121±0.019 0.090±0.056 0.019±0.004 
Ser 0.906±0.042 1.156±0.154 0.084±0.013 0.050±0.006 0.038±0.005 0.026±0.004 
Tau 16.70±1.46 18.30±2.44 17.00±1.12 14.17±0.42 0.387±0.074 0.321±0.041 
Thr 1.290±0.129 1.337±0.302 0.352±0.078(3) 0.485±0.082 0.451±0.078(3) 0.184±0.037(3)* 
Try 0.058±0.005 0.031±0.005* 0.042±0.008 0.021±0.004 0.060±0.003 0.027±0.003(3)* 
Tyr 0.064±0.004 0.053±0.005 0.055±0.006 0.058±0.005 0.036±0.002 0.019±0.005(3)* 
Val 0.145±0.016 0.100±0.013 0.054±0.017 0.032±0.007 0.118±0.016 0.099±0.025 
TFAA 55.12±4.00 69.69±4.29* 26.62±1.76 25.27±3.06(3) 2.786±0.310 2.267±0.241 

Values are means ± S.E.M. (N=4, unless indicated otherwise in parentheses).

n.d., not detectable.

*

Significantly different from control values (P<0.05).

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