Table 2.

Summary of changes in expression of glutamine synthetase protein(western blots) and mRNA (ribonuclease protection assays) in various tissues of B. sinensis after exposure to NH4Cl for 48 h

Western blots (arbitrary numbers)Ribonuclease protection assays (GSase/actin ratio)
TissueControl+NH4ClIncrease (-fold)Control+NH4ClIncrease (-fold)
Liver 1662±231 9753±1881* 5.9 0.049±0.004 0.988±0.201* 20.2 
Intestine 1642±558 2021±375 1.2 0.187±0.048 1.326±0.194* 7.1 
Stomach 4532±2451 4617±188 1.0 0.300±0.051 0.676±0.062* 2.3 
Muscle 10 539±2479 12 500±1855 1.2 0.077±0.036 0.678±0.084* 8.8 
Western blots (arbitrary numbers)Ribonuclease protection assays (GSase/actin ratio)
TissueControl+NH4ClIncrease (-fold)Control+NH4ClIncrease (-fold)
Liver 1662±231 9753±1881* 5.9 0.049±0.004 0.988±0.201* 20.2 
Intestine 1642±558 2021±375 1.2 0.187±0.048 1.326±0.194* 7.1 
Stomach 4532±2451 4617±188 1.0 0.300±0.051 0.676±0.062* 2.3 
Muscle 10 539±2479 12 500±1855 1.2 0.077±0.036 0.678±0.084* 8.8 

GSase, glutamine synthetase.

Values are means ± 1 S.E.M. (N=5); 1N=4.

*

Significantly different from the control values (P<0.05).

View Large
Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal
This site uses cookies. By continuing to use our website, you are agreeing to our privacy policy.
Accept