The Company of Biologists

Table 1.

Red muscle glycolytic intermediates, glycerol, intramuscular triacylglycerol, free CoA and short chain fatty acyl-carnitine concentrations in control fish and fish swum at 30, 60 and 90% U_crit for up to 240 min

		Swimming speed
		30% U_crit					60% U_crit				90% U_crit
Metabolite	Control	2 min	15 min	240 min	2 min	15 min	240 min	2 min	15 min	45 min
Fru 6-P	0.11±0.02	0.06±0.02^a	0.12±0.03^a	0.01±0.0^{^*,a}	0.12±0.05^a	0.09±0.02^a	0.09±0.05^a	0.25±0.04^{^*,b}	0.15±0.03^a	0.08±0.01
Gly 3-P	0.04±0.03	0.03±0.03^a	0.12±0.07^a	0.09±0.09^a	0.32±0.09^a,b	1.07±0.39^{^*,b}	0.13±0.07^a	1.17±0.12^{^*,b,c}	2.82±0.29^{^*,c}	2.19±0.58^{^*}
Glycerol	2.03±0.37	0.93±0.19^a	1.58±0.25^a	1.94±0.41^a	1.27±0.45^a	1.71±0.37^a	2.22±0.50^a	1.19±0.55^a	2.57±0.53^a	5.20±0.96^{^*}
IMTG	207±10 (8)	198±12^a (8)	200±11^a (6)	181±12^a (8)	212±16^a (4)	197±22^a (5)	203±24^a (7)	201±8^a (7)	197±8^a (8)	216±24 (7)
CoA-SH	53.6±5.4	48.5±7.3^a	56.8±6.0^a	50.0±3.8^a	57.6±6.2^a	37.4±4.6^{^*,b}	49.1±4.9^a	46.7±3.8^a	38.4±5.1^{^*,b}	46.8±4.4
SCFA-Car	13±25	49±12^a	61±9^a	33±10^a	49±4^a	10±8^a	31±19^a	1±1^a	1±1^b	30±19

		Swimming speed
		30% U_crit					60% U_crit				90% U_crit
Metabolite	Control	2 min	15 min	240 min	2 min	15 min	240 min	2 min	15 min	45 min
Fru 6-P	0.11±0.02	0.06±0.02^a	0.12±0.03^a	0.01±0.0^{^*,a}	0.12±0.05^a	0.09±0.02^a	0.09±0.05^a	0.25±0.04^{^*,b}	0.15±0.03^a	0.08±0.01
Gly 3-P	0.04±0.03	0.03±0.03^a	0.12±0.07^a	0.09±0.09^a	0.32±0.09^a,b	1.07±0.39^{^*,b}	0.13±0.07^a	1.17±0.12^{^*,b,c}	2.82±0.29^{^*,c}	2.19±0.58^{^*}
Glycerol	2.03±0.37	0.93±0.19^a	1.58±0.25^a	1.94±0.41^a	1.27±0.45^a	1.71±0.37^a	2.22±0.50^a	1.19±0.55^a	2.57±0.53^a	5.20±0.96^{^*}
IMTG	207±10 (8)	198±12^a (8)	200±11^a (6)	181±12^a (8)	212±16^a (4)	197±22^a (5)	203±24^a (7)	201±8^a (7)	197±8^a (8)	216±24 (7)
CoA-SH	53.6±5.4	48.5±7.3^a	56.8±6.0^a	50.0±3.8^a	57.6±6.2^a	37.4±4.6^{^*,b}	49.1±4.9^a	46.7±3.8^a	38.4±5.1^{^*,b}	46.8±4.4
SCFA-Car	13±25	49±12^a	61±9^a	33±10^a	49±4^a	10±8^a	31±19^a	1±1^a	1±1^b	30±19

Values are means ± S.E.M., N=8 except where indicated in parentheses.

Fru 6-P, fructose 6-phosphate; Gly 3-P, glycerol 3-phosphate; IMTG,intramuscular triacylglycerol; CoA-SH, free CoA; SCFA-carnitine, short chain fatty acyl-carnitine.

Fru 6-P, Gly 3-P and glycerol, values are μmol g^-1 wet tissue; IMTG is expressed in glycerol units (1 glycerol=3 fatty acids from IMTG); CoA-SH and SCFA-Car values are nmol g^-1 wet tissue.

Value significantly different from control (P<0.05); within a time period, values with different letters are significantly different.

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