Table 3.

Predicted effects on fertilization of changes in egg target size and density

Egg
SizeSize and density
Method/trialExperiment numberNlogFC50Proportion changelogFC50Proportion change
Acidification  1.25 0.51 1.49 0.70 
  1.01 0.37 1.26 0.51 
  1.42 0.74 1.66 1.01 
  1.19 0.82 1.44 1.13 
  0.70 0.34 0.95 0.47 
  1.38 0.57 1.62 0.79 
  1.04 0.44 1.29 0.61 
  0.64 0.41 0.89 0.56 
  0.83 1.00 1.07 1.38 
 10  0.81 0.33 1.06 0.46 
Mean±S.E.M.  10 1.03±0.09 0.55±0.07 1.27±0.09 0.76±0.10 
Neutral/mechanical 11  0.68 0.53 0.93 0.68 
 12  1.21 0.61 1.46 0.83 
 13  0.72 0.51 0.97 0.62 
 14  1.29 0.55 1.53 0.75 
 15  0.89 0.36 1.14 0.50 
Mean±S.E.M.  0.96±0.12 0.51±0.04 1.20±0.12 0.68±0.06 
All trials       
Mean±S.E.M.  15 1.00±0.07 0.54±0.05 1.25±0.07 0.73±0.07 
Egg
SizeSize and density
Method/trialExperiment numberNlogFC50Proportion changelogFC50Proportion change
Acidification  1.25 0.51 1.49 0.70 
  1.01 0.37 1.26 0.51 
  1.42 0.74 1.66 1.01 
  1.19 0.82 1.44 1.13 
  0.70 0.34 0.95 0.47 
  1.38 0.57 1.62 0.79 
  1.04 0.44 1.29 0.61 
  0.64 0.41 0.89 0.56 
  0.83 1.00 1.07 1.38 
 10  0.81 0.33 1.06 0.46 
Mean±S.E.M.  10 1.03±0.09 0.55±0.07 1.27±0.09 0.76±0.10 
Neutral/mechanical 11  0.68 0.53 0.93 0.68 
 12  1.21 0.61 1.46 0.83 
 13  0.72 0.51 0.97 0.62 
 14  1.29 0.55 1.53 0.75 
 15  0.89 0.36 1.14 0.50 
Mean±S.E.M.  0.96±0.12 0.51±0.04 1.20±0.12 0.68±0.06 
All trials       
Mean±S.E.M.  15 1.00±0.07 0.54±0.05 1.25±0.07 0.73±0.07 

logFC50 is the sperm concentration predicted by the model to achieve 50% fertilization for eggs that have the size-independent properties of intact eggs but a size (or size and density) change equivalent to removal of the jelly coat.

`% change' gives the proportion of the total change in logFC50 from intact to coat-free eggs (data in Table 2) that is accounted for by the predicted logFC50. Mean values of % change were not significantly different between the two removal methods for size(t=0.52, P<0.61) or size and density (t=0.73, P<0.48).

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