The Company of Biologists

Table 2.

Estimates of the parameter β (mm³ s^-1), the ratio β/β₀, and logFC₅₀(μl^-1) for intact and coat-free eggs based on fertilization trials

			Egg
			Intact					Coat-free
Method/trial	Experiment number	N	β (×10^-4)	β/β₀ (×10^-2)	logFC₅₀	β (×10^-4)	β/β₀ (×10^-2)	logFC₅₀
Acidification	1		2.75	1.85	0.59	0.11	0.43	1.88
	2		4.73	3.17	0.35	0.05	0.24	2.14
	3		1.86	1.25	0.75	0.18	0.73	1.65
	4		3.10	2.08	0.53	0.38	1.51	1.33
	5		9.58	6.43	0.04	0.09	0.36	1.96
	6		2.03	1.36	0.72	0.11	0.44	1.87
	7		4.39	2.95	0.38	0.11	0.44	1.87
	8		11.01	7.39	-0.02	0.20	0.81	1.61
	9		7.22	4.85	0.17	1.22	4.87	0.83
	10		7.50	5.03	0.15	0.06	0.24	2.13
Mean±S.E.M.		10	5.42±1.02	3.64±0.69	0.37±0.09	0.25±0.11	1.01±0.45	1.73±0.13
Neutral/Mechanical	11		10.11	6.79	0.02	0.44	1.77	1.26
	12		3.00	2.01	0.55	0.19	0.75	1.64
	13		9.24	6.20	0.06	0.36	1.44	1.36
	14		2.50	1.68	0.63	0.12	0.48	1.84
	15		6.27	4.21	0.23	0.07	0.30	2.04
Mean±S.E.M.		5	6.22±1.56	4.18±1.05	0.30±0.12	0.24±0.07	0.95±0.28	1.63±0.15
All trials
Mean±S.E.M.		15	5.69±0.83	3.82±0.41	0.34±0.05	0.25±0.76	0.99±0.35	1.69±0.10

			Egg
			Intact					Coat-free
Method/trial	Experiment number	N	β (×10^-4)	β/β₀ (×10^-2)	logFC₅₀	β (×10^-4)	β/β₀ (×10^-2)	logFC₅₀
Acidification	1		2.75	1.85	0.59	0.11	0.43	1.88
	2		4.73	3.17	0.35	0.05	0.24	2.14
	3		1.86	1.25	0.75	0.18	0.73	1.65
	4		3.10	2.08	0.53	0.38	1.51	1.33
	5		9.58	6.43	0.04	0.09	0.36	1.96
	6		2.03	1.36	0.72	0.11	0.44	1.87
	7		4.39	2.95	0.38	0.11	0.44	1.87
	8		11.01	7.39	-0.02	0.20	0.81	1.61
	9		7.22	4.85	0.17	1.22	4.87	0.83
	10		7.50	5.03	0.15	0.06	0.24	2.13
Mean±S.E.M.		10	5.42±1.02	3.64±0.69	0.37±0.09	0.25±0.11	1.01±0.45	1.73±0.13
Neutral/Mechanical	11		10.11	6.79	0.02	0.44	1.77	1.26
	12		3.00	2.01	0.55	0.19	0.75	1.64
	13		9.24	6.20	0.06	0.36	1.44	1.36
	14		2.50	1.68	0.63	0.12	0.48	1.84
	15		6.27	4.21	0.23	0.07	0.30	2.04
Mean±S.E.M.		5	6.22±1.56	4.18±1.05	0.30±0.12	0.24±0.07	0.95±0.28	1.63±0.15
All trials
Mean±S.E.M.		15	5.69±0.83	3.82±0.41	0.34±0.05	0.25±0.76	0.99±0.35	1.69±0.10

β, rate constant of fertilization; β₀, rate constant of collision; logFC₅₀, log-transformed sperm concentration at which 50% fertilization is achieved.

Values of β were derived from a non-linear regression fit of the fertilization kinetics model to the data for each trial.

The ratio β/β₀ was calculated using values forβ ₀ of 0.0149 and 0.0025 mm³s^-1 for intact and coat-free eggs, respectively.

The concentration FC₅₀ was estimated by iteration usingβ and other model parameters (Table 1).

Mean values of β for coat-free eggs did not differ significantly between the two removal methods (t=0.11, P<0.09).

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