Table 4.

Rho1 does not promote rescue of pbl loss-of-function mutant in a PblΔBRCT overexpression background

GenotypeEve-positive hemisegmentss.d.n
pbl3/pbl3 1.7 1.7 128 
PblΔBRCT, pbl3/pbl3 10.3 3.5 102 
Rho1, pbl3/PblΔBRCTpbl3 10.2 4.0 99 
GenotypeEve-positive hemisegmentss.d.n
pbl3/pbl3 1.7 1.7 128 
PblΔBRCT, pbl3/pbl3 10.3 3.5 102 
Rho1, pbl3/PblΔBRCTpbl3 10.2 4.0 99 

Mean values and standard deviations (s.d.) of the number of Eve-positive hemisegments are shown for pbl3 homozygous embryos and pbl3 homozygotes expressing wild-type Rho1 protein in the mesoderm using twi::Gal4 (n=number of embryos examined). The crosses employed for this experiment were different from the experiment described in Table 3 and resulted in slightly better rescue of the pbl3 phenotype by PblΔBRCT (see Materials and methods). The number of Eve-positive hemisegments between pbl3 homozygotes expressing PblΔBRCT and pbl3 homozygotes expressing PblΔBRCT and Rho1 was largely unimpaired.

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