The Company of Biologists

Table 1.

The 23 Madd mutants recovered in our screen for genes required for muscle arm extension

	Complementation group	Allele	LG^{^*}	Map position^{^*}	Failed to complement^{^†}	Homolog	Mutation^{^‡}
1	gex-2	tr116	IV	<–16	ok1603	Sra1/p140/Cyfip1	R420Stop (c5009t)
2	madd-2^{^§}	tr64	V
3		tr96
4		tr101
5		tr103
6		tr113
7		tr129
8	unc-33	tr114	IV	–5 < +1	e204	Crmp2/Dpysl2	R502H (g6504a)
9	unc-40	tr63	I	–0.7 < 1.0	n324	Dcc/neogenin	Intron 6 splice donor (g4869a)
10		tr115		> –1.7	n324		W1107Stop (g8867a)
11		tr121		–4.5 < 1.0	n324		Exon 8 splice donor/D426N (g5765a)
12	unc-51	tr126	V	+19.4 > +25.2	e369	Ulk2	I59T (t1240c)
13	unc-54	tr112	I	LGI	e190	MHC-B/Myh7	–
14		tr124		LGI	e190		–
15	unc-60B	tr125	V	LGV	su158	Cofilin/ADF	G44E (g2241a)
16		tr50		–19.1 < –17.6	su158		–
17	unc-73	tr117	I	–4.5 < 1.0	e936		E1335K (g9486a)
18	unc-93	tr120^sd	III	–7.4 < –2	e1500	UNC-93	G388R (g2476a)
19	unc-95	tr61	I	LGI	su33	UNC-95	Intron 1 splice acceptor (g1693a)
20	–	tr98^d	I	–6.2 < –4.5	–	–	–
21	–	tr105	I	+4 < +23.5	–	–	–
22	–	tr119	I	+1.9 < +4	–	–	–
23	–	tr123	I	–4.8 < –1.6	–	–	–

	Complementation group	Allele	LG^{^*}	Map position^{^*}	Failed to complement^{^†}	Homolog	Mutation^{^‡}
1	gex-2	tr116	IV	<–16	ok1603	Sra1/p140/Cyfip1	R420Stop (c5009t)
2	madd-2^{^§}	tr64	V
3		tr96
4		tr101
5		tr103
6		tr113
7		tr129
8	unc-33	tr114	IV	–5 < +1	e204	Crmp2/Dpysl2	R502H (g6504a)
9	unc-40	tr63	I	–0.7 < 1.0	n324	Dcc/neogenin	Intron 6 splice donor (g4869a)
10		tr115		> –1.7	n324		W1107Stop (g8867a)
11		tr121		–4.5 < 1.0	n324		Exon 8 splice donor/D426N (g5765a)
12	unc-51	tr126	V	+19.4 > +25.2	e369	Ulk2	I59T (t1240c)
13	unc-54	tr112	I	LGI	e190	MHC-B/Myh7	–
14		tr124		LGI	e190		–
15	unc-60B	tr125	V	LGV	su158	Cofilin/ADF	G44E (g2241a)
16		tr50		–19.1 < –17.6	su158		–
17	unc-73	tr117	I	–4.5 < 1.0	e936		E1335K (g9486a)
18	unc-93	tr120^sd	III	–7.4 < –2	e1500	UNC-93	G388R (g2476a)
19	unc-95	tr61	I	LGI	su33	UNC-95	Intron 1 splice acceptor (g1693a)
20	–	tr98^d	I	–6.2 < –4.5	–	–	–
21	–	tr105	I	+4 < +23.5	–	–	–
22	–	tr119	I	+1.9 < +4	–	–	–
23	–	tr123	I	–4.8 < –1.6	–	–	–

The linkage group (LG) (i.e. chromosome) and map position are shown. Only the linkage group is shown if mapping did not proceed beyond bulk segregant analysis

†

The allele used in the complementation test is shown

‡

The mutant residue is shown followed by the mutant nucleotide in brackets,which is relative to the adenine of the predicted start codon in the genomic sequence (WormBase release 187). For tr117 and tr125, the mutant nucleotides are with respect to F55C7.7b and C38C3.5c.1, respectively. tr63 carries a mutation in the invariant first base of the splice donor of intron 6, which is likely to result in the translation of 11 additional codons within intron 6 before a stop codon is reached, truncating the protein between the second and third IG domains. Similarly, tr121is mutant in the last nucleotide of exon 8 and may also disrupt splicing(Farrer et al., 2002). For those aberrantly spliced tr121 transcripts, a stop codon is present 99 codons into intron 8, resulting in a predicted truncated protein between the fourth IG domain and the first fibronectin type III domain. For those transcripts without altered splicing, a D426N mutation is created between the fourth IG domain and the first fibronectin type III domain

Details of the six madd-2 alleles isolated in our screen will be presented elsewhere

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