The Company of Biologists

Table 3.

Six3 regulation of signaling pathway components

	Six3-mRNA/glycerol			Six3-MO/Δcadherin			Six3-MO/glycerol
Gene	Array log₂ signal	QPCR ΔCt	Array log₂ signal	QPCR ΔCt	Array log₂ signal	QPCR ΔCt
Nodal	−1.3	−2.1	2.3	3	−0.2	−0.1
Lefty	−1.6	−2.7	2.4	2.2	−1.6	−2
Chordin	−2	−2.4	2.5	2.7	−2	−2
BMP	−0.72	−0.1	−0.5	−2	−1.1	−0.8
Wnt1	−2.1	−5.4	2.6	2.1	0.6	0.2
Wnt4	−4.1	−5.1	4	5.9	0.7	0.6
Wnt8	−1.5	−3.5	2.4	1.8	0.5	0.8
Wnt16	−2.5	−5.5	1.6	1.4	0.9	0.5
delta^{^*}			−0.9	−3.9
fgf^{^*}			−2	−2.4
fgfrl^{^*}			−1.8	−1.5
Frizzled5/8^{^*}			−2.6	−2.1

	Six3-mRNA/glycerol			Six3-MO/Δcadherin			Six3-MO/glycerol
Gene	Array log₂ signal	QPCR ΔCt	Array log₂ signal	QPCR ΔCt	Array log₂ signal	QPCR ΔCt
Nodal	−1.3	−2.1	2.3	3	−0.2	−0.1
Lefty	−1.6	−2.7	2.4	2.2	−1.6	−2
Chordin	−2	−2.4	2.5	2.7	−2	−2
BMP	−0.72	−0.1	−0.5	−2	−1.1	−0.8
Wnt1	−2.1	−5.4	2.6	2.1	0.6	0.2
Wnt4	−4.1	−5.1	4	5.9	0.7	0.6
Wnt8	−1.5	−3.5	2.4	1.8	0.5	0.8
Wnt16	−2.5	−5.5	1.6	1.4	0.9	0.5
delta^{^*}			−0.9	−3.9
fgf^{^*}			−2	−2.4
fgfrl^{^*}			−1.8	−1.5
Frizzled5/8^{^*}			−2.6	−2.1

Measured in cadherin-misexpressing embryos to eliminate Six3 effects in endomesoderm.