. | % Defective segments . | . | |
---|---|---|---|
Genotype . | Stage 15 . | Stage 17 . | |
Nrx-IV4304/Nrx-IV4304 | 93 (27/29) | 90 (29/32) | |
elav-Gal4/+; UAS-Nrx-IV/+; Nrx-IV4304/TM6 | 3 (1/34) | 4 (1/28) | |
elav-Gal4/+; UAS-Nrx-IV/+;Nrx-IV4304/Nrx-IV4304 | 22 (7/32) | 83 (25/30) | |
UAS-Nrx-IV/+; slit-Gal4 Nrx-IV4304/TM6 | 3 (1/33) | 0 (0/30) | |
UAS-Nrx-IV/+; slit-Gal4 Nrx-IV4304/Nrx-IV4304 | 92 (22/24) | 96 (24/25) |
. | % Defective segments . | . | |
---|---|---|---|
Genotype . | Stage 15 . | Stage 17 . | |
Nrx-IV4304/Nrx-IV4304 | 93 (27/29) | 90 (29/32) | |
elav-Gal4/+; UAS-Nrx-IV/+; Nrx-IV4304/TM6 | 3 (1/34) | 4 (1/28) | |
elav-Gal4/+; UAS-Nrx-IV/+;Nrx-IV4304/Nrx-IV4304 | 22 (7/32) | 83 (25/30) | |
UAS-Nrx-IV/+; slit-Gal4 Nrx-IV4304/TM6 | 3 (1/33) | 0 (0/30) | |
UAS-Nrx-IV/+; slit-Gal4 Nrx-IV4304/Nrx-IV4304 | 92 (22/24) | 96 (24/25) |
Values in parentheses indicate the number of affected segments/total number of segments examined. MG migration was assayed using MG position, shape and anti-Wrapper staining. Additionally, in the slit-Gal4 experiments,anti-Nrx-IV staining identified MG expressing UAS-Nrx-IV. Homozygous Nrx-IV mutants were identified by the absence of Nrx-IV staining from the epidermis.